Low genetic differentiation among sardine populations, Sardinella aurita, from eastern Venezuela

In view of the great importance of Sardinella aurita as a fisheries resource in the Caribbean region, we studied the genetic differentiation among the populations from Mochima, Gulf of Cariaco and Morro de Puerto Santo, eastern Venezuela, and compared the levels of variation and population subdivisi...

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Main Authors: De-Donato, M, Mimbela-de-Loroño, I, Ramírez, I, Marín, B
Format: Online
Language:eng
Published: Iniversidad Autónoma de Baja California 2005
Online Access:https://www.cienciasmarinas.com.mx/index.php/cmarinas/article/view/44
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spelling oai:cienciasmarinas.com.mx:article-442019-04-23T18:47:24Z Low genetic differentiation among sardine populations, Sardinella aurita, from eastern Venezuela Baja diferenciación genética entre poblaciones de sardina, Sardinella aurita, del oriente venezolano De-Donato, M Mimbela-de-Loroño, I Ramírez, I Marín, B PCR-RFLP mitochondrial DNA population genetics Clupeidae PCR-RFLP ADN mitocondrial genética de poblaciones Clupeidae In view of the great importance of Sardinella aurita as a fisheries resource in the Caribbean region, we studied the genetic differentiation among the populations from Mochima, Gulf of Cariaco and Morro de Puerto Santo, eastern Venezuela, and compared the levels of variation and population subdivision with populations from the Mediterranean and Atlantic coast of Africa (previously reported). For this, we extracted DNA from 15 muscle samples for each population. A fragment containing the control region of the mitochondrial DNA was amplified and the restriction patterns were determined using restriction fragment length polymorphism (RFLP) analysis with the enzymes Taq I, Eco RI, Hae III and Alu I. The amplification of this region produced a fragment approximately 1000 bp long or, rarely, one of approximately 1100 bp long. No further difference was found through the RFLP analysis. The comparison of the Venezuelan populations produced FST values under 0.06 and the analysis of molecular variance showed no significant differences between them. This is an indication of very low genetic differentiation among these populations, so it is not possible to discard the presence of a panmictic population in this region. The comparison of the Venezuelan and Mediterranean populations produced very low FST values, while the comparison of the former with African populations from Mauritania, Senegal, Ivory Coast and Ghana produced FST values ten times larger. Debido a la gran importancia de Sardinella aurita como recurso pesquero de la región del Caribe, se estudió la diferenciación genética entre las poblaciones de Mochima, Golfo de Cariaco y Morro de Puerto Santo, en el oriente de Venezuela, y se compararon los niveles de variación y subdivisión poblacional con las poblaciones del Mediterráneo y la costa Atlántica de África, anteriormente reportadas. Para esto, se obtuvieron 15 individuos de cada una de tres poblaciones, utilizando una muestra de músculo para extraer el ADN total. Se amplificó un fragmento que incluye la región de control del ADN mitocondrial y se determinaron los patrones de restricción por medio del análisis por RFLP con las enzimas Taq I, Eco RI, Hae III y Alu I. La amplificación de esta región produjo un fragmento de aproximadamente 1000 bp o, rara vez, uno de 1100 pb. El análisis de RFLP demostró la presencia de sólo un haplotipo con las dos variantes de tamaño. El FST resultante del análisis de las diferencias entre poblaciones venezolanas fue menor de 0.06 y el AMOVA demostró que no existían diferencias significativas entre ellas. Estos resultados concuerdan con la presencia de poca diferenciación genética entre estas poblaciones, por lo que no se puede descartar la presencia de una población panmíctica en esta región. Una comparación de las poblaciones venezolanas con las poblaciones del Mediterráneo y África, reportadas con anterioridad, produjeron valores de FST muy bajos entre las venezolanas y las del Mediterráneo, mientras que las comparaciones entre las de Mauritania, Senegal, Costa de Marfil y Ghana produjeron valores de FST diez veces mayores. Iniversidad Autónoma de Baja California 2005-03-06 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article Artículo Arbitrado application/pdf https://www.cienciasmarinas.com.mx/index.php/cmarinas/article/view/44 10.7773/cm.v31i3.44 Ciencias Marinas; Vol. 31 No. 3 (2005); 529-535 Ciencias Marinas; Vol. 31 Núm. 3 (2005); 529-535 2395-9053 0185-3880 eng https://www.cienciasmarinas.com.mx/index.php/cmarinas/article/view/44/26
institution Ciencias Marinas
collection OJS
language eng
format Online
author De-Donato, M
Mimbela-de-Loroño, I
Ramírez, I
Marín, B
spellingShingle De-Donato, M
Mimbela-de-Loroño, I
Ramírez, I
Marín, B
Low genetic differentiation among sardine populations, Sardinella aurita, from eastern Venezuela
author_facet De-Donato, M
Mimbela-de-Loroño, I
Ramírez, I
Marín, B
author_sort De-Donato, M
title Low genetic differentiation among sardine populations, Sardinella aurita, from eastern Venezuela
title_short Low genetic differentiation among sardine populations, Sardinella aurita, from eastern Venezuela
title_full Low genetic differentiation among sardine populations, Sardinella aurita, from eastern Venezuela
title_fullStr Low genetic differentiation among sardine populations, Sardinella aurita, from eastern Venezuela
title_full_unstemmed Low genetic differentiation among sardine populations, Sardinella aurita, from eastern Venezuela
title_sort low genetic differentiation among sardine populations, sardinella aurita, from eastern venezuela
description In view of the great importance of Sardinella aurita as a fisheries resource in the Caribbean region, we studied the genetic differentiation among the populations from Mochima, Gulf of Cariaco and Morro de Puerto Santo, eastern Venezuela, and compared the levels of variation and population subdivision with populations from the Mediterranean and Atlantic coast of Africa (previously reported). For this, we extracted DNA from 15 muscle samples for each population. A fragment containing the control region of the mitochondrial DNA was amplified and the restriction patterns were determined using restriction fragment length polymorphism (RFLP) analysis with the enzymes Taq I, Eco RI, Hae III and Alu I. The amplification of this region produced a fragment approximately 1000 bp long or, rarely, one of approximately 1100 bp long. No further difference was found through the RFLP analysis. The comparison of the Venezuelan populations produced FST values under 0.06 and the analysis of molecular variance showed no significant differences between them. This is an indication of very low genetic differentiation among these populations, so it is not possible to discard the presence of a panmictic population in this region. The comparison of the Venezuelan and Mediterranean populations produced very low FST values, while the comparison of the former with African populations from Mauritania, Senegal, Ivory Coast and Ghana produced FST values ten times larger.
publisher Iniversidad Autónoma de Baja California
publishDate 2005
url https://www.cienciasmarinas.com.mx/index.php/cmarinas/article/view/44
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