Histological validation of the gonadal maturation stages of female jumbo squid (Dosidicus gigas) in the Gulf of California, Mexico.

The oocyte structure of jumbo squid (Dosidicus gigas) female gonads was analyzed to validate morphochromatic sexual maturation stages based on a six-phase scale. Samples were obtained fortnightly from the Santa Rosalía area, Baja California Sur, from June 2001 to November 2002. Histological preparat...

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Main Authors: Díaz-Uribe, JG, Hernández-Herrera, A, Morales-Bojórquez, E, Martínez-Aguilar, S, Suárez-Higuera, MC, Hernández-López, A
Format: Online
Language:eng
Published: Iniversidad Autónoma de Baja California 2006
Online Access:https://www.cienciasmarinas.com.mx/index.php/cmarinas/article/view/68
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spelling oai:cienciasmarinas.com.mx:article-682019-04-08T20:19:07Z Histological validation of the gonadal maturation stages of female jumbo squid (Dosidicus gigas) in the Gulf of California, Mexico. Validación histológica de los estadios de madurez gonádica de las hembras de calamar gigante (Dosidicus gigas) en el Golfo de California, México Díaz-Uribe, JG Hernández-Herrera, A Morales-Bojórquez, E Martínez-Aguilar, S Suárez-Higuera, MC Hernández-López, A Dosidicus gigas jumbo squid gonad maturation maturity index Gulf of California Dosidicus gigas calamar gigante maduración gonádica índice de madurez Golfo de California The oocyte structure of jumbo squid (Dosidicus gigas) female gonads was analyzed to validate morphochromatic sexual maturation stages based on a six-phase scale. Samples were obtained fortnightly from the Santa Rosalía area, Baja California Sur, from June 2001 to November 2002. Histological preparations were obtained from each gonad to count the number of oocytes at each level of development. The frequency of oocytes at each level was then used to estimate a maturity index (MI) to assess the correspondence between morphochromatic maturation stages and the oocyte structure in the gonad tissue. The histological analysis showed that morphochromatic stages I, II and III were ruled by oocytes in developmental level 2 or less (i.e., secondary growth has not yet begun), and thus correspond to sexually immature juveniles. Stages IV, V and VI were ruled by oocytes in more advanced developmental levels and correspond to mature or maturing adults. According to the analysis of variance, the morphochromatic maturity scale allowed sexually immature individuals to be readily and reliably distinguished from mature ones. A detailed analysis of MI confidence intervals showed that each morphochromatic maturity stage had a specific oocyte structure, except for stages II and III, which presented morphological differences. Se analizó la estructura de ovocitos en las gónadas de hembras de calamar gigante (Dosidicus gigas), para validar los estadios morfocromáticos de madurez sexual, basados en una escala de seis etapas. Las muestras fueron obtenidas quincenalmente en el área de Santa Rosalía, Baja California Sur, entre junio del 2001 y noviembre del 2002. De cada gónada se obtuvieron cortes histológicos para contar el número de ovocitos en cada nivel de desarrollo. Con la frecuencia de ovocitos en cada nivel de desarrollo, se estimó el índice de madurez (IM) para evaluar la correspondencia de cada estadio de madurez morfocromática con la estructura de ovocitos en el tejido de la gónada. El análisis histológico indica que los primeros tres estadios de madurez morfocromática (I–III) predominan ovocitos en un nivel de desarrollo 2 o inferior, en los cuales no inicia todavía el crecimiento secundario y por lo tanto corresponden a organismos juveniles sexualmente inmaduros. En los otros tres estadios (IV–VI) predominan ovocitos en niveles de desarrollo más avanzados que corresponden a adultos maduros o en proceso de maduración. De acuerdo con el análisis de varianza, esta escala de madurez permite distinguir de manera confiable y expedita a los organismos sexualmente inmaduros de los maduros. Un análisis más detallado de los intervalos de confianza del IM, indica que cada estadio de madurez morfocromática presenta una estructura de ovocitos distinta excepto entre los estadios II y III en los cuales las diferencias se observan más bien a nivel morfológico. Iniversidad Autónoma de Baja California 2006-02-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article Artículo Arbitrado application/pdf https://www.cienciasmarinas.com.mx/index.php/cmarinas/article/view/68 10.7773/cm.v32i1.68 Ciencias Marinas; Vol. 32 No. 1 (2006); 23-31 Ciencias Marinas; Vol. 32 Núm. 1 (2006); 23-31 2395-9053 0185-3880 eng https://www.cienciasmarinas.com.mx/index.php/cmarinas/article/view/68/50
institution Ciencias Marinas
collection OJS
language eng
format Online
author Díaz-Uribe, JG
Hernández-Herrera, A
Morales-Bojórquez, E
Martínez-Aguilar, S
Suárez-Higuera, MC
Hernández-López, A
spellingShingle Díaz-Uribe, JG
Hernández-Herrera, A
Morales-Bojórquez, E
Martínez-Aguilar, S
Suárez-Higuera, MC
Hernández-López, A
Histological validation of the gonadal maturation stages of female jumbo squid (Dosidicus gigas) in the Gulf of California, Mexico.
author_facet Díaz-Uribe, JG
Hernández-Herrera, A
Morales-Bojórquez, E
Martínez-Aguilar, S
Suárez-Higuera, MC
Hernández-López, A
author_sort Díaz-Uribe, JG
title Histological validation of the gonadal maturation stages of female jumbo squid (Dosidicus gigas) in the Gulf of California, Mexico.
title_short Histological validation of the gonadal maturation stages of female jumbo squid (Dosidicus gigas) in the Gulf of California, Mexico.
title_full Histological validation of the gonadal maturation stages of female jumbo squid (Dosidicus gigas) in the Gulf of California, Mexico.
title_fullStr Histological validation of the gonadal maturation stages of female jumbo squid (Dosidicus gigas) in the Gulf of California, Mexico.
title_full_unstemmed Histological validation of the gonadal maturation stages of female jumbo squid (Dosidicus gigas) in the Gulf of California, Mexico.
title_sort histological validation of the gonadal maturation stages of female jumbo squid (dosidicus gigas) in the gulf of california, mexico.
description The oocyte structure of jumbo squid (Dosidicus gigas) female gonads was analyzed to validate morphochromatic sexual maturation stages based on a six-phase scale. Samples were obtained fortnightly from the Santa Rosalía area, Baja California Sur, from June 2001 to November 2002. Histological preparations were obtained from each gonad to count the number of oocytes at each level of development. The frequency of oocytes at each level was then used to estimate a maturity index (MI) to assess the correspondence between morphochromatic maturation stages and the oocyte structure in the gonad tissue. The histological analysis showed that morphochromatic stages I, II and III were ruled by oocytes in developmental level 2 or less (i.e., secondary growth has not yet begun), and thus correspond to sexually immature juveniles. Stages IV, V and VI were ruled by oocytes in more advanced developmental levels and correspond to mature or maturing adults. According to the analysis of variance, the morphochromatic maturity scale allowed sexually immature individuals to be readily and reliably distinguished from mature ones. A detailed analysis of MI confidence intervals showed that each morphochromatic maturity stage had a specific oocyte structure, except for stages II and III, which presented morphological differences.
publisher Iniversidad Autónoma de Baja California
publishDate 2006
url https://www.cienciasmarinas.com.mx/index.php/cmarinas/article/view/68
_version_ 1715723930697728000