First step in the evaluation of the effects of Prestige oil on the shore environment: Availability, bioaccumulation and DNA damage

The Prestige oil tanker shipwrecked off the coast of Galicia (Spain) in November 2002, spilling nearly 63,000 tons of heavy oil, classified by the International Agency for Research on Cancer as possible human carcinogen. In this work, mussels (Mytilus galloprovincialis) were exposed to Prestige oil...

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Main Authors: Laffon, B, Aldao, I, Pérez-Cadahia, B, Pásaro, E, Méndez, J
Format: info:eu-repo/semantics/article
Language:eng
Published: Iniversidad Autónoma de Baja California 2006
Subjects:
PAH
Online Access:https://www.cienciasmarinas.com.mx/index.php/cmarinas/article/view/1091
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id repositorioinstitucional-20.500.12930-7415
record_format dspace
institution Repositorio Institucional
collection DSpace
language eng
topic Mytilus galloprovincialis
Prestige
PAH
DNA damage
bioaccumulation
Mytilus galloprovincialis
Prestige
PAH
daño en el ADN
bioacumulación
spellingShingle Mytilus galloprovincialis
Prestige
PAH
DNA damage
bioaccumulation
Mytilus galloprovincialis
Prestige
PAH
daño en el ADN
bioacumulación
Laffon, B
Aldao, I
Pérez-Cadahia, B
Pásaro, E
Méndez, J
First step in the evaluation of the effects of Prestige oil on the shore environment: Availability, bioaccumulation and DNA damage
description The Prestige oil tanker shipwrecked off the coast of Galicia (Spain) in November 2002, spilling nearly 63,000 tons of heavy oil, classified by the International Agency for Research on Cancer as possible human carcinogen. In this work, mussels (Mytilus galloprovincialis) were exposed to Prestige oil in the laboratory (1.5 and 3 ppm). Samples of seawater and mussel tissues were taken on days 7 and 14 of the experiment to determine the availability and bioaccumulation of the polycyclic aromatic hydrocarbons (PAH) contained in the oil and the DNA damage induced by the exposure using the comet assay. Seawater was renewed on day 14 and new samples were taken on day 21 to analyze the recovery ability of the mussels. Total PAH (TPAH) contents in seawater from the exposure tanks were higher than in the control tank, and this content was higher in the lower oil-dose tank due to the tendency of PAH to link to particles in water. Exposed mussels had much higher TPAH levels than controls, increasing with time even during the recovery phase. The correlation coefficient obtained demonstrated a clear environmental dose/internal dose relationship. Significant increases in DNA damage were observed in oil-exposed individuals in relation to controls, similar in the two doses tested. The DNA damage was constant during the exposure period but increased during the recovery time, reflecting the strand breakage during the DNA repair processes. The results obtained have shown the importance of determining the presence of pollutants in the environment, their bioaccumulation in organic tissues and their effects on the exposed organisms, in order to perform an integrated approach to evaluate the impact of contamination events in the aquatic environment.
format info:eu-repo/semantics/article
author Laffon, B
Aldao, I
Pérez-Cadahia, B
Pásaro, E
Méndez, J
author_facet Laffon, B
Aldao, I
Pérez-Cadahia, B
Pásaro, E
Méndez, J
author_sort Laffon, B
title First step in the evaluation of the effects of Prestige oil on the shore environment: Availability, bioaccumulation and DNA damage
title_short First step in the evaluation of the effects of Prestige oil on the shore environment: Availability, bioaccumulation and DNA damage
title_full First step in the evaluation of the effects of Prestige oil on the shore environment: Availability, bioaccumulation and DNA damage
title_fullStr First step in the evaluation of the effects of Prestige oil on the shore environment: Availability, bioaccumulation and DNA damage
title_full_unstemmed First step in the evaluation of the effects of Prestige oil on the shore environment: Availability, bioaccumulation and DNA damage
title_sort first step in the evaluation of the effects of prestige oil on the shore environment: availability, bioaccumulation and dna damage
publisher Iniversidad Autónoma de Baja California
publishDate 2006
url https://www.cienciasmarinas.com.mx/index.php/cmarinas/article/view/1091
_version_ 1792610339072770048
spelling repositorioinstitucional-20.500.12930-74152023-05-09T14:30:57Z First step in the evaluation of the effects of Prestige oil on the shore environment: Availability, bioaccumulation and DNA damage Primer paso en la evaluación de los efectos del fuel del Prestige sobre el medio ambiente marino: Disponibilidad, bioacumulación y daño en el ADN Laffon, B Aldao, I Pérez-Cadahia, B Pásaro, E Méndez, J Mytilus galloprovincialis Prestige PAH DNA damage bioaccumulation Mytilus galloprovincialis Prestige PAH daño en el ADN bioacumulación The Prestige oil tanker shipwrecked off the coast of Galicia (Spain) in November 2002, spilling nearly 63,000 tons of heavy oil, classified by the International Agency for Research on Cancer as possible human carcinogen. In this work, mussels (Mytilus galloprovincialis) were exposed to Prestige oil in the laboratory (1.5 and 3 ppm). Samples of seawater and mussel tissues were taken on days 7 and 14 of the experiment to determine the availability and bioaccumulation of the polycyclic aromatic hydrocarbons (PAH) contained in the oil and the DNA damage induced by the exposure using the comet assay. Seawater was renewed on day 14 and new samples were taken on day 21 to analyze the recovery ability of the mussels. Total PAH (TPAH) contents in seawater from the exposure tanks were higher than in the control tank, and this content was higher in the lower oil-dose tank due to the tendency of PAH to link to particles in water. Exposed mussels had much higher TPAH levels than controls, increasing with time even during the recovery phase. The correlation coefficient obtained demonstrated a clear environmental dose/internal dose relationship. Significant increases in DNA damage were observed in oil-exposed individuals in relation to controls, similar in the two doses tested. The DNA damage was constant during the exposure period but increased during the recovery time, reflecting the strand breakage during the DNA repair processes. The results obtained have shown the importance of determining the presence of pollutants in the environment, their bioaccumulation in organic tissues and their effects on the exposed organisms, in order to perform an integrated approach to evaluate the impact of contamination events in the aquatic environment. El petrolero Prestige se hundió frente a la costa gallega en noviembre de 2002, vertiendo unas 63,000 toneladas de fuel pesado, clasificado por la Agencia Internacional para Investigación en Cáncer como posible carcinógeno humano. En este trabajo se expusieron mejillones (Mytilus galloprovincialis) a fuel del Prestige en el laboratorio (1.5 y 3 ppm). Se tomaron muestras de agua de mar y tejidos de mejillón a los 7 y 14 días para determinar la disponibilidad y la bioacumulación de los hidrocarburos aromáticos policíclicos (PAH) contenidos en el fuel, y el daño en el ADN inducido por la exposición mediante el ensayo del cometa. El agua de mar fue renovada en el día 14 y se tomaron nuevas muestras el día 21 para analizar la capacidad de recuperación de los mejillones. El contenido total de PAHs (TPAH) en el agua de los tanques expuestos resultó mayor que en el tanque control, siendo superior en el tanque con la menor dosis debido a la gran tendencia que los PAHs presentan a unirse partículas en el agua. Los mejillones expuestos presentaron mayores niveles de TPAH que los controles, incrementándose en el tiempo durante la fase de recuperación. El coeficiente de correlación obtenido demostró una clara relación dosis ambiental/dosis interna. Se observó incremento significativo en el daño en el ADN en los individuos expuestos, similar en las dos dosis probadas. El daño en el ADN fue constante durante los periodos de exposición y recuperación, reflejo de las roturas de cadena durante los procesos de reparación del ADN. Los resultados obtenidos muestran la importancia de determinar la presencia de los polucionantes en el ambiente, su bioacumulación en los tejidos y sus efectos en los organismos expuestos, para realizar una aproximación integrada en la evaluación de los efectos de los eventos de contaminación en el ambiente marino. 2006-08-21 2021-06-03T03:55:15Z 2021-06-03T03:55:15Z info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article Artículo Arbitrado https://www.cienciasmarinas.com.mx/index.php/cmarinas/article/view/1091 10.7773/cm.v32i22.1091 https://hdl.handle.net/20.500.12930/7415 eng https://www.cienciasmarinas.com.mx/index.php/cmarinas/article/view/1091/1920 application/pdf Iniversidad Autónoma de Baja California Ciencias Marinas; Vol. 32 No. 2B (2006); 389-399 Ciencias Marinas; Vol. 32 Núm. 2B (2006); 389-399 2395-9053 0185-3880