Maturation and fertilization in vitro of Pteria sterna (Gould, 1851, Mollusca)

Maturation and fertilization in vitro of Pteria sterna (Gould, 1851, Mollusca)

 P. sterna oocytes cannot be fertilized in vitro unless they are obtained by inducing spawning. The present work was undertaken to develop a simple method to induce germinal vesicle breakdown that would enable the oocytes to be fertilized without prior spawning. We found that Pteria sterna...

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Autori principali: Harris, C, Fierro, S, De-La-Cerda, A, Domínguez, D, Echanove, JJ, Fierro, I, Garay, K, García, B, González, JC, Lara, R, López, AC, Martínez, J, Montalvo, M, Nieto, R, Oceguera, A, Orozco, C, Sánchez, G, Torres, AL, Varela, S, Vital, V, Zavala, JO, Gould, MC
Natura: Online
Lingua:eng
Pubblicazione: Iniversidad Autónoma de Baja California 2002
Accesso online:https://www.cienciasmarinas.com.mx/index.php/cmarinas/article/view/218
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spelling oai:cienciasmarinas.com.mx:article-2182019-04-30T23:15:50Z Maturation and fertilization in vitro of Pteria sterna (Gould, 1851, Mollusca) Maduración y fecundación in vitro de los ovocitos de Pteria sterna (Gould, 1851, Mollusca) Harris, C Fierro, S De-La-Cerda, A Domínguez, D Echanove, JJ Fierro, I Garay, K García, B González, JC Lara, R López, AC Martínez, J Montalvo, M Nieto, R Oceguera, A Orozco, C Sánchez, G Torres, AL Varela, S Vital, V Zavala, JO Gould, MC oocytes germinal vesicle breakdown serotonin ammonium chloride fertilization ovocitos ruptura de Ia vesicula germinal serotonina cloruro de amonio fecundaci6n  P. sterna oocytes cannot be fertilized in vitro unless they are obtained by inducing spawning. The present work was undertaken to develop a simple method to induce germinal vesicle breakdown that would enable the oocytes to be fertilized without prior spawning. We found that Pteria sterna oocytes removed from the ovaries are induced to undergo germinal vesicle breakdown (GVBD) by 10 mM NH4Cl in seawater, and proceed to metaphase I of meiosis, at which stage they can be fertilized and will develop at least as far as "D" larvae. On the other hand, ovarian oocytes exposed to 2 × 10–5 M serotonin in seawater remained in prophase and did not undergo GVBD. Ammonium chloride treatment provides a simple, inexpensive procedure that can be useful in studies of fertilization and development, and for attempts to repopulate this exploited species. Los ovocitos de P. sterna no pueden ser fecundados in vitro sin una previa inducción al desove, de manera que, en el presente trabajo se pretende desarrollar un método sencillo para inducir la ruptura de vesículas germinales, permitiendo así la fecundación sin un desove previo. Encontramos que los ovocitos extraidos de la gónada de Pteria sterna presentan ruptura de la vesicular germinal (RVG) ante el agente estimulante NH4Cl (10–2 M), es decir, se activan los ovocitos (a partir de la profase I) y continúan la meiosis hasta la metafase I, lo que permite la fecundación y el desarrollo de los organismos hasta larvas "D". Contrario a lo anterior, la serotonina (2 × 10–5 M) no propicia la maduración (RVG) en los ovocitos de la especie mencionada. Esta información puede ser utilizada para la fecundación in vitro y en intentos de repoblación del bivalvo P. sterna, así como en futuros estudios sobre su reproducción. Iniversidad Autónoma de Baja California 2002-03-06 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article Artículo Arbitrado application/pdf https://www.cienciasmarinas.com.mx/index.php/cmarinas/article/view/218 10.7773/cm.v28i2.218 Ciencias Marinas; Vol. 28 No. 2 (2002); 149-156 Ciencias Marinas; Vol. 28 Núm. 2 (2002); 149-156 2395-9053 0185-3880 eng https://www.cienciasmarinas.com.mx/index.php/cmarinas/article/view/218/1551
institution Ciencias Marinas
collection OJS
language eng
format Online
author Harris, C
Fierro, S
De-La-Cerda, A
Domínguez, D
Echanove, JJ
Fierro, I
Garay, K
García, B
González, JC
Lara, R
López, AC
Martínez, J
Montalvo, M
Nieto, R
Oceguera, A
Orozco, C
Sánchez, G
Torres, AL
Varela, S
Vital, V
Zavala, JO
Gould, MC
spellingShingle Harris, C
Fierro, S
De-La-Cerda, A
Domínguez, D
Echanove, JJ
Fierro, I
Garay, K
García, B
González, JC
Lara, R
López, AC
Martínez, J
Montalvo, M
Nieto, R
Oceguera, A
Orozco, C
Sánchez, G
Torres, AL
Varela, S
Vital, V
Zavala, JO
Gould, MC
Maturation and fertilization in vitro of Pteria sterna (Gould, 1851, Mollusca)
author_facet Harris, C
Fierro, S
De-La-Cerda, A
Domínguez, D
Echanove, JJ
Fierro, I
Garay, K
García, B
González, JC
Lara, R
López, AC
Martínez, J
Montalvo, M
Nieto, R
Oceguera, A
Orozco, C
Sánchez, G
Torres, AL
Varela, S
Vital, V
Zavala, JO
Gould, MC
author_sort Harris, C
title Maturation and fertilization in vitro of Pteria sterna (Gould, 1851, Mollusca)
title_short Maturation and fertilization in vitro of Pteria sterna (Gould, 1851, Mollusca)
title_full Maturation and fertilization in vitro of Pteria sterna (Gould, 1851, Mollusca)
title_fullStr Maturation and fertilization in vitro of Pteria sterna (Gould, 1851, Mollusca)
title_full_unstemmed Maturation and fertilization in vitro of Pteria sterna (Gould, 1851, Mollusca)
title_sort maturation and fertilization in vitro of pteria sterna (gould, 1851, mollusca)
description  P. sterna oocytes cannot be fertilized in vitro unless they are obtained by inducing spawning. The present work was undertaken to develop a simple method to induce germinal vesicle breakdown that would enable the oocytes to be fertilized without prior spawning. We found that Pteria sterna oocytes removed from the ovaries are induced to undergo germinal vesicle breakdown (GVBD) by 10 mM NH4Cl in seawater, and proceed to metaphase I of meiosis, at which stage they can be fertilized and will develop at least as far as "D" larvae. On the other hand, ovarian oocytes exposed to 2 × 10–5 M serotonin in seawater remained in prophase and did not undergo GVBD. Ammonium chloride treatment provides a simple, inexpensive procedure that can be useful in studies of fertilization and development, and for attempts to repopulate this exploited species.
publisher Iniversidad Autónoma de Baja California
publishDate 2002
url https://www.cienciasmarinas.com.mx/index.php/cmarinas/article/view/218
_version_ 1715723943660224512

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