Characterization of the immune response of the north scallop Argopecten purpuratus (Lamarck, 1819) (Mollusca: Bivalvia)

Characterization of the immune response of the north scallop Argopecten purpuratus (Lamarck, 1819) (Mollusca: Bivalvia)

The immune response in mollusks has an important role as a defense against bacteriae, fungi, protozoa and metazoans. The hemocytes are responsible for generating different kinds of innate responses such as: phagocytosis, encapsulation and production of cytotoxic substances and antibacterial peptides...

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Autores principales: González, M, Arenas, G
Formato: Online
Lenguaje:eng
Publicado: Iniversidad Autónoma de Baja California 2002
Acceso en línea:https://www.cienciasmarinas.com.mx/index.php/cmarinas/article/view/227
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id oai:cienciasmarinas.com.mx:article-227
record_format ojs
institution Ciencias Marinas
collection OJS
language eng
format Online
author González, M
Arenas, G
spellingShingle González, M
Arenas, G
Characterization of the immune response of the north scallop Argopecten purpuratus (Lamarck, 1819) (Mollusca: Bivalvia)
author_facet González, M
Arenas, G
author_sort González, M
title Characterization of the immune response of the north scallop Argopecten purpuratus (Lamarck, 1819) (Mollusca: Bivalvia)
title_short Characterization of the immune response of the north scallop Argopecten purpuratus (Lamarck, 1819) (Mollusca: Bivalvia)
title_full Characterization of the immune response of the north scallop Argopecten purpuratus (Lamarck, 1819) (Mollusca: Bivalvia)
title_fullStr Characterization of the immune response of the north scallop Argopecten purpuratus (Lamarck, 1819) (Mollusca: Bivalvia)
title_full_unstemmed Characterization of the immune response of the north scallop Argopecten purpuratus (Lamarck, 1819) (Mollusca: Bivalvia)
title_sort characterization of the immune response of the north scallop argopecten purpuratus (lamarck, 1819) (mollusca: bivalvia)
description The immune response in mollusks has an important role as a defense against bacteriae, fungi, protozoa and metazoans. The hemocytes are responsible for generating different kinds of innate responses such as: phagocytosis, encapsulation and production of cytotoxic substances and antibacterial peptides. Through light microscopy we estimated the hemocyte number using a Neubauer chamber. Over hemocytes monolayers we measured the phagocytic ability on yeasts. The haemolytic activity on rat's erythrocytes was tested by hemoglobin release and the production of anion superoxide was quantified by the reduction of NBT (Nitro Blue Tetrazolium). We determined that the immune system from Argopecten purpuratus was defined by two types of hemocytes: hyalinocytes and granulocytes. The number of hemocytes circulating in the hemolymph showed a seasonal variation, having a greater increase during winter than in autumn. The hemocytes phagocytized yeast in the absence of plasma when they were opsonized, showing a remarkable increase in phagocytosis. Plasma and hemocytes have an equivalent hemolytic ability against rat?s erythrocytes. The hemocytes present production of anion superoxide that increases considerably over the control with the use of zymosan and, decreases with the use of the superoxide dismutase. The results show that hemocytes from Argopecten purpuratus are able to display different defense mechanisms during the cellular response that could be used as tools to evaluate the immune system of mollusks in the near future.
publisher Iniversidad Autónoma de Baja California
publishDate 2002
url https://www.cienciasmarinas.com.mx/index.php/cmarinas/article/view/227
_version_ 1715723944818900992
spelling oai:cienciasmarinas.com.mx:article-2272019-04-30T19:43:21Z Characterization of the immune response of the north scallop Argopecten purpuratus (Lamarck, 1819) (Mollusca: Bivalvia) Caracterización de la respuesta inmune en el ostión del norte Argopecten purpuratus (Lamarck, 1819) (Mollusca: Bivalvia) González, M Arenas, G Mollusks immune response hemocytes Argopecten purpuratus Moluscos respuesta inmune hemocitos Argopecten purpuratus The immune response in mollusks has an important role as a defense against bacteriae, fungi, protozoa and metazoans. The hemocytes are responsible for generating different kinds of innate responses such as: phagocytosis, encapsulation and production of cytotoxic substances and antibacterial peptides. Through light microscopy we estimated the hemocyte number using a Neubauer chamber. Over hemocytes monolayers we measured the phagocytic ability on yeasts. The haemolytic activity on rat's erythrocytes was tested by hemoglobin release and the production of anion superoxide was quantified by the reduction of NBT (Nitro Blue Tetrazolium). We determined that the immune system from Argopecten purpuratus was defined by two types of hemocytes: hyalinocytes and granulocytes. The number of hemocytes circulating in the hemolymph showed a seasonal variation, having a greater increase during winter than in autumn. The hemocytes phagocytized yeast in the absence of plasma when they were opsonized, showing a remarkable increase in phagocytosis. Plasma and hemocytes have an equivalent hemolytic ability against rat?s erythrocytes. The hemocytes present production of anion superoxide that increases considerably over the control with the use of zymosan and, decreases with the use of the superoxide dismutase. The results show that hemocytes from Argopecten purpuratus are able to display different defense mechanisms during the cellular response that could be used as tools to evaluate the immune system of mollusks in the near future. Los mecanismos de defensa en moluscos cumplen una importante función defensiva contra bacterias, hongos, protozoos y metazoos. Los hemocitos son los responsables de generar diferentes tipos de respuestas innatas tales como: fagocitosis, encapsulación, producción de sustancias citotóxicas y péptidos antibacterianos. Por microcopía de luz, se estimó el tipo y número de hemocitos utilizando una cámara de Neubauer, en monocapa, se midió la capacidad fagocítica sobre levaduras, la actividad hemolítica sobre eritrocitos de rata y la producción de anión superóxido fue cuantificada midiendo la reducción de NBT en forma citoquímica. Se determinó que la hemolinfa de Argopecten purpuratus contiene hemocitos del tipo hialino y granular. El número de hemocitos circulantes en la hemolinfa de los ostiones presentó una variación estacional mostrando un aumento significativo en invierno, respecto de otoño. Los hemocitos fagocitaron levaduras en ausencia de plasma, cuando se opsonizaron con el suero, la fagocitosis aumentó significativamente. Tanto el suero como los hemocitos presentaron igual capacidad hemolítica sobre eritrocitos de rata. Los hemocitos presentaron producción de anión superóxido que, al utilizar zimosan, aumentó considerablemente respecto al control, mientras que en presencia de la enzima superóxido dismutasa, ésta disminuyó. Los resultados obtenidos muestran que los hemocitos de A. purpuratus son capaces de presentar variados mecanismos defensivos de respuesta celular que podrían ser utilizados en el futuro como instrumentos para evaluar la condición de los mecanismos de defensa, y proyectarlos sobre el estado de salud de los individuos en cultivo. Iniversidad Autónoma de Baja California 2002-03-06 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article Artículo Arbitrado application/pdf https://www.cienciasmarinas.com.mx/index.php/cmarinas/article/view/227 10.7773/cm.v28i3.227 Ciencias Marinas; Vol. 28 No. 3 (2002); 247-255 Ciencias Marinas; Vol. 28 Núm. 3 (2002); 247-255 2395-9053 0185-3880 eng https://www.cienciasmarinas.com.mx/index.php/cmarinas/article/view/227/194

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