Spatiotemporal distribution of Vibrio parahaemolyticus in relation to environmental parameters in a coastal lagoon on the Pacific coast of northwestern Mexico

Spatiotemporal distribution of Vibrio parahaemolyticus in relation to environmental parameters in a coastal lagoon on the Pacific coast of northwestern Mexico

Vibrio parahaemolyticus has been responsible for the increasing number of diarrhea cases in Sinaloa, Mexico, since 2003. We investigated the presence, distribution, and content of V. parahaemolyticus toxigenic genes detected in water, zooplankton, and sediment samples in relation to environmental va...

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Autores principales: Rivas-Montaño, Ana M, Luis-Villaseñor, Irasema E, Piña-Váldez, Pablo, Gómez-Gil, Bruno, Lizárraga-Partida, Marcial L
Formato: Online
Lenguaje:eng
Publicado: Iniversidad Autónoma de Baja California 2018
Acceso en línea:https://www.cienciasmarinas.com.mx/index.php/cmarinas/article/view/2772
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spelling oai:cienciasmarinas.com.mx:article-27722024-03-03T15:00:59Z Spatiotemporal distribution of Vibrio parahaemolyticus in relation to environmental parameters in a coastal lagoon on the Pacific coast of northwestern Mexico Distribución espaciotemporal de Vibrio parahaemolyticus con relación a los parámetros ambientales en una laguna costera en la costa del Pacífico del noroeste de México Rivas-Montaño, Ana M Luis-Villaseñor, Irasema E Piña-Váldez, Pablo Gómez-Gil, Bruno Lizárraga-Partida, Marcial L Vibrio parahaemolyticus has been responsible for the increasing number of diarrhea cases in Sinaloa, Mexico, since 2003. We investigated the presence, distribution, and content of V. parahaemolyticus toxigenic genes detected in water, zooplankton, and sediment samples in relation to environmental variables in Caimanero Lagoon (Mazatlán, Sinaloa). Samples were analyzed by PCR to detect the presence of V. parahaemolyticus and its toxigenic factors. Of all the samples analyzed, 57.5% tested positive for thermolabile hemolysin (tlh), a gene indicative of the species. The thermostable direct hemolysin (tdh) and tdh-related hemolysin (trh) genes, which are both pathogenicity markers of this species, were detected in 9% and 6% of the samples, respectively. The orf8 fragment, which has been recently detected in pandemic strains (O3:K6), was detected in 11% of the samples. Vibrio parahaemolyticus was detected more frequently in zooplankton, with the highest incidence observed in February. Salinity was positively correlated with V. parahaemolyticus; however, no correlation was found between V. parahaemolyticus and temperature. The presence of toxigenic V. parahaemolyticus during the different seasons indicates the need to maintain continuous sanitary inspection of fish products from Caimanero Lagoon. Vibrio parahaemolyticus ha sido responsable del creciente número de casos de diarrea en Sinaloa, México, desde 2003. Investigamos la presencia, la distribución y el contenido de los genes toxigénicos de V. parahaemolyticus que fueron detectados en muestras de agua, zooplancton y sedimento y su relación con las variables ambientales en la laguna Caimanero (Mazatlán, Sinaloa). Las muestras fueron analizadas por reacción en cadena de la polimerasa para detectar la presencia de V. parahaemolyticus y sus factores toxigénicos. De todas las muestras analizadas, el 57.5% resultaron positivas para la hemolisina termolábil (tlh), un gen indicativo de la especie. Los genes de la hemolisina directa termoestable (tdh) y la hemolisina relacionada a la tdh (trh), considerados marcadores de la patogenicidad de la especie, fueron detectados en el 9% y el 6% de las muestras, respectivamente. El fragmento orf8, que recientemente se ha detectado en cepas pandémicas (O3:K6), se detectó en el 11% de las muestras. Vibrio parahaemolyticus fue detectado con mayor frecuencia en el zooplancton, con la incidencia más alta observada en febrero. La salinidad se correlacionó positivamente con V. parahaemolyticus; sin embargo, no se registró correlación entre V. parahaemolyticus y la temperatura. La presencia de V. parahaemolyticus toxigénico durante las diferentes temporadas indica la necesidad de mantener la vigilancia sanitaria continua de los productos pesqueros de la laguna Caimanero. Iniversidad Autónoma de Baja California 2018-09-28 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article Artículo Arbitrado application/pdf text/xml text/xml https://www.cienciasmarinas.com.mx/index.php/cmarinas/article/view/2772 10.7773/cm.v44i3.2772 Ciencias Marinas; Vol. 44 No. 3 (2018); 141–153 Ciencias Marinas; Vol. 44 Núm. 3 (2018); 141–153 2395-9053 0185-3880 eng https://www.cienciasmarinas.com.mx/index.php/cmarinas/article/view/2772/420420446 https://www.cienciasmarinas.com.mx/index.php/cmarinas/article/view/2772/420421008 https://www.cienciasmarinas.com.mx/index.php/cmarinas/article/view/2772/420420622 Copyright (c) 2018 Ciencias Marinas https://creativecommons.org/licenses/by/4.0
institution Ciencias Marinas
collection OJS
language eng
format Online
author Rivas-Montaño, Ana M
Luis-Villaseñor, Irasema E
Piña-Váldez, Pablo
Gómez-Gil, Bruno
Lizárraga-Partida, Marcial L
spellingShingle Rivas-Montaño, Ana M
Luis-Villaseñor, Irasema E
Piña-Váldez, Pablo
Gómez-Gil, Bruno
Lizárraga-Partida, Marcial L
Spatiotemporal distribution of Vibrio parahaemolyticus in relation to environmental parameters in a coastal lagoon on the Pacific coast of northwestern Mexico
author_facet Rivas-Montaño, Ana M
Luis-Villaseñor, Irasema E
Piña-Váldez, Pablo
Gómez-Gil, Bruno
Lizárraga-Partida, Marcial L
author_sort Rivas-Montaño, Ana M
title Spatiotemporal distribution of Vibrio parahaemolyticus in relation to environmental parameters in a coastal lagoon on the Pacific coast of northwestern Mexico
title_short Spatiotemporal distribution of Vibrio parahaemolyticus in relation to environmental parameters in a coastal lagoon on the Pacific coast of northwestern Mexico
title_full Spatiotemporal distribution of Vibrio parahaemolyticus in relation to environmental parameters in a coastal lagoon on the Pacific coast of northwestern Mexico
title_fullStr Spatiotemporal distribution of Vibrio parahaemolyticus in relation to environmental parameters in a coastal lagoon on the Pacific coast of northwestern Mexico
title_full_unstemmed Spatiotemporal distribution of Vibrio parahaemolyticus in relation to environmental parameters in a coastal lagoon on the Pacific coast of northwestern Mexico
title_sort spatiotemporal distribution of vibrio parahaemolyticus in relation to environmental parameters in a coastal lagoon on the pacific coast of northwestern mexico
description Vibrio parahaemolyticus has been responsible for the increasing number of diarrhea cases in Sinaloa, Mexico, since 2003. We investigated the presence, distribution, and content of V. parahaemolyticus toxigenic genes detected in water, zooplankton, and sediment samples in relation to environmental variables in Caimanero Lagoon (Mazatlán, Sinaloa). Samples were analyzed by PCR to detect the presence of V. parahaemolyticus and its toxigenic factors. Of all the samples analyzed, 57.5% tested positive for thermolabile hemolysin (tlh), a gene indicative of the species. The thermostable direct hemolysin (tdh) and tdh-related hemolysin (trh) genes, which are both pathogenicity markers of this species, were detected in 9% and 6% of the samples, respectively. The orf8 fragment, which has been recently detected in pandemic strains (O3:K6), was detected in 11% of the samples. Vibrio parahaemolyticus was detected more frequently in zooplankton, with the highest incidence observed in February. Salinity was positively correlated with V. parahaemolyticus; however, no correlation was found between V. parahaemolyticus and temperature. The presence of toxigenic V. parahaemolyticus during the different seasons indicates the need to maintain continuous sanitary inspection of fish products from Caimanero Lagoon.
publisher Iniversidad Autónoma de Baja California
publishDate 2018
url https://www.cienciasmarinas.com.mx/index.php/cmarinas/article/view/2772
_version_ 1792606973718429696

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