Taxonomic profile of bacterial communities detected with 16S-rRNA in mature phototrophic and heterotrophic marine biofilms used for aquaculture

Taxonomic profile of bacterial communities detected with 16S-rRNA in mature phototrophic and heterotrophic marine biofilms used for aquaculture

Bacterial diversity of phototrophic (PAb) and heterotrophic (Hb) biofilms was studied over time (0, 15 and 30 days of culture) using high throughput sequencing and considering the V3 and V4 hypervariable regions of the 16S rRNA gene. Bacterial composition in terms of relative abundance and diversity...

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Principais autores: Martínez-Córdova, Luis Rafael, Martínez-Porchas, Marcel, Vargas-Albores, Francisco, Miranda-Baeza, Anselmo, Coelho-Emerenciano, Mauricio, Porchas-Cornejo, Marco Antonio, Cortes-Jacinto, Edilmar, Mazorra-Manzano, Miguel Ángel
Formato: Online
Idioma:eng
Publicado em: Iniversidad Autónoma de Baja California 2018
Acesso em linha:https://www.cienciasmarinas.com.mx/index.php/cmarinas/article/view/2829
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spelling oai:cienciasmarinas.com.mx:article-28292024-03-03T15:44:11Z Taxonomic profile of bacterial communities detected with 16S-rRNA in mature phototrophic and heterotrophic marine biofilms used for aquaculture Perfil taxonómico de comunidades bacterianas detectadas con 16S-ARNr en biopelículas marinas autotróficas y heterotróficas utilizadas en la acuacultura Martínez-Córdova, Luis Rafael Martínez-Porchas, Marcel Vargas-Albores, Francisco Miranda-Baeza, Anselmo Coelho-Emerenciano, Mauricio Porchas-Cornejo, Marco Antonio Cortes-Jacinto, Edilmar Mazorra-Manzano, Miguel Ángel Bacterial diversity of phototrophic (PAb) and heterotrophic (Hb) biofilms was studied over time (0, 15 and 30 days of culture) using high throughput sequencing and considering the V3 and V4 hypervariable regions of the 16S rRNA gene. Bacterial composition in terms of relative abundance and diversity showed slight changes during the trial. Proteobacteria was the most abundant phylum in both biofilms during the experimental period. Chlamidiae-Verrucomicrobia, Bacteriodetes, and Planctomycetes were also abundant phyla in the PAb, whereas Planctomycetes, Bacteriodetes, Actinobacteria, and Chlamydiae were the abundant phyla in the Hb. Of the reads assigned up to species level, a total of 27 heterotrophic and autotrophic species were detected in both biofilms, most of them associated with the metabolism of nitrogenous and sulfurous metabolites and organic matter, and the rest with the structural functions in the biofilm. This is the first time some of these species have been reportedly detected in these biofilms or in the marine environment. Results suggest complex interaction networks in microbial conglomerates formed in biofilms, in which bacterial populations seem to play important metabolic and physiological roles. Se estudió la diversidad de bacterias de biopelículas fototróficas (PAb) y heterotróficas (Hb) a través del tiempo (0, 15 y 30 días de cultivo) por medio de secuenciación masiva, considerando las regiones hipervariables V3 y V4 del gen 16S ARNr. La composición bacteriana mostró pequeños cambios durante el ensayo en términos de abundancia relativa y diversidad. Proteobacteria fue el filo más abundante en ambas biopelículas durante el experimento. Chlamidiae-Verrucomicrobia, Bacteriodetes y Planctomycetes también fueron filos abundantes en PAb, mientras que Planctomycetes, Bacteriodetes, Actinobacteria y Chlamydiae se registraron en Hb. De las lecturas asignadas a nivel de especie, un total de 27 especies autrotróficas y heterotróficas fueron detectadas en ambas biopelículas, la mayoría de ellas asociadas al metabolismo de compuestos nitrogenados y azufrados y a materia orgánica, y las restantes asociadas a las funciones estructurales en las biopelículas. Esta es la primera vez que se reporta la detección de algunas de estas especies en estas biopelículas e incluso en el ambiente marino. Los resultados sugieren la existencia de complejas redes de interacción en los conglomerados microbianos formados en las bipelículas, en las cuales las poblaciones bacterianas parecen jugar papeles metabólicos y fisiológicos importantes. Iniversidad Autónoma de Baja California 2018-12-20 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article Artículo Arbitrado application/pdf text/xml text/xml https://www.cienciasmarinas.com.mx/index.php/cmarinas/article/view/2829 10.7773/cm.v44i4.2829 Ciencias Marinas; Vol. 44 No. 4 (2018); 251–266 Ciencias Marinas; Vol. 44 Núm. 4 (2018); 251–266 2395-9053 0185-3880 eng https://www.cienciasmarinas.com.mx/index.php/cmarinas/article/view/2829/420420454 https://www.cienciasmarinas.com.mx/index.php/cmarinas/article/view/2829/420421015 https://www.cienciasmarinas.com.mx/index.php/cmarinas/article/view/2829/420420630 Copyright (c) 2018 Ciencias Marinas https://creativecommons.org/licenses/by/4.0
institution Ciencias Marinas
collection OJS
language eng
format Online
author Martínez-Córdova, Luis Rafael
Martínez-Porchas, Marcel
Vargas-Albores, Francisco
Miranda-Baeza, Anselmo
Coelho-Emerenciano, Mauricio
Porchas-Cornejo, Marco Antonio
Cortes-Jacinto, Edilmar
Mazorra-Manzano, Miguel Ángel
spellingShingle Martínez-Córdova, Luis Rafael
Martínez-Porchas, Marcel
Vargas-Albores, Francisco
Miranda-Baeza, Anselmo
Coelho-Emerenciano, Mauricio
Porchas-Cornejo, Marco Antonio
Cortes-Jacinto, Edilmar
Mazorra-Manzano, Miguel Ángel
Taxonomic profile of bacterial communities detected with 16S-rRNA in mature phototrophic and heterotrophic marine biofilms used for aquaculture
author_facet Martínez-Córdova, Luis Rafael
Martínez-Porchas, Marcel
Vargas-Albores, Francisco
Miranda-Baeza, Anselmo
Coelho-Emerenciano, Mauricio
Porchas-Cornejo, Marco Antonio
Cortes-Jacinto, Edilmar
Mazorra-Manzano, Miguel Ángel
author_sort Martínez-Córdova, Luis Rafael
title Taxonomic profile of bacterial communities detected with 16S-rRNA in mature phototrophic and heterotrophic marine biofilms used for aquaculture
title_short Taxonomic profile of bacterial communities detected with 16S-rRNA in mature phototrophic and heterotrophic marine biofilms used for aquaculture
title_full Taxonomic profile of bacterial communities detected with 16S-rRNA in mature phototrophic and heterotrophic marine biofilms used for aquaculture
title_fullStr Taxonomic profile of bacterial communities detected with 16S-rRNA in mature phototrophic and heterotrophic marine biofilms used for aquaculture
title_full_unstemmed Taxonomic profile of bacterial communities detected with 16S-rRNA in mature phototrophic and heterotrophic marine biofilms used for aquaculture
title_sort taxonomic profile of bacterial communities detected with 16s-rrna in mature phototrophic and heterotrophic marine biofilms used for aquaculture
description Bacterial diversity of phototrophic (PAb) and heterotrophic (Hb) biofilms was studied over time (0, 15 and 30 days of culture) using high throughput sequencing and considering the V3 and V4 hypervariable regions of the 16S rRNA gene. Bacterial composition in terms of relative abundance and diversity showed slight changes during the trial. Proteobacteria was the most abundant phylum in both biofilms during the experimental period. Chlamidiae-Verrucomicrobia, Bacteriodetes, and Planctomycetes were also abundant phyla in the PAb, whereas Planctomycetes, Bacteriodetes, Actinobacteria, and Chlamydiae were the abundant phyla in the Hb. Of the reads assigned up to species level, a total of 27 heterotrophic and autotrophic species were detected in both biofilms, most of them associated with the metabolism of nitrogenous and sulfurous metabolites and organic matter, and the rest with the structural functions in the biofilm. This is the first time some of these species have been reportedly detected in these biofilms or in the marine environment. Results suggest complex interaction networks in microbial conglomerates formed in biofilms, in which bacterial populations seem to play important metabolic and physiological roles.
publisher Iniversidad Autónoma de Baja California
publishDate 2018
url https://www.cienciasmarinas.com.mx/index.php/cmarinas/article/view/2829
_version_ 1792606974721916928

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