A simple method for the genetic identification of commercially important species in the Opisthonema genus Gill, 1861 in the southern Gulf of California

A simple method for the genetic identification of commercially important species in the Opisthonema genus Gill, 1861 in the southern Gulf of California

The polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique was used to identify 3 species in the genus Opisthonema (Opisthonema bulleri, Opisthonema medirastre, and Opisthonema libertate) in the southern Gulf of California. Two specific primer pairs were designed to...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Pérez-Quiñonez, Carlos Iván, Quiñonez-Velázquez, Casimiro, García-Rodríguez, Francisco Javier
Formato: Online
Lenguaje:eng
Publicado: Iniversidad Autónoma de Baja California 2020
Acceso en línea:https://www.cienciasmarinas.com.mx/index.php/cmarinas/article/view/3059
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
id oai:cienciasmarinas.com.mx:article-3059
record_format ojs
spelling oai:cienciasmarinas.com.mx:article-30592024-02-28T01:02:14Z A simple method for the genetic identification of commercially important species in the Opisthonema genus Gill, 1861 in the southern Gulf of California Un método sencillo para la identificación genética de especies comercialmente importantes del género Opisthonema Gill, 1861 en el sur del golfo de California Pérez-Quiñonez, Carlos Iván Quiñonez-Velázquez, Casimiro García-Rodríguez, Francisco Javier PCR-RFLP Opisthonema restriction enzymes COI gene identification PCR-RFLP Opisthonema enzimas de restricción gen COI identificación The polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique was used to identify 3 species in the genus Opisthonema (Opisthonema bulleri, Opisthonema medirastre, and Opisthonema libertate) in the southern Gulf of California. Two specific primer pairs were designed to amplify the COI mitochondrial gene and 3 restriction enzymes (HpaII, RsaI, and AvaII) were selected to compare the RFLP patterns among the species. Different polymorphism levels were detected among samples. Easily analyzed specific restriction profiles were created, which differentiated unequivocally among the 3 target species. The approach undertaken here using PCR-RFLP is relatively cheap, fast, and robust, providing a useful technique for the identification of species in the genus Opisthonema. Se aplicó la técnica de reacción en cadena de la polimerasa-polimorfismos de longitud de fragmentos de restricción (PCR-RFLP, por sus siglas en inglés) para identificar 3 especies del género Opisthonema (Opisthonema bulleri, Opisthonema medirastre y Opisthonema libertate) en la región sur del golfo de California. Se diseñaron 2 pares de iniciadores específicos para amplificar el gen mitocondrial COI y se seleccionaron 3 enzimas de restricción (HpaII, RsaI y AvaII) para comparar los patrones de RFLP entre las especies. Diferentes niveles de polimorfismo fueron detectados entre las muestras. Se generaron perfiles de restricción específicos fáciles de analizar, los cuales pudieran distinguir de manera inequívoca las 3 especies objetivo. El enfoque llevado a cabo aquí usando PCR-RFLP es relativamente barato, rápido y robusto, y proporciona una técnica útil para la identificación de las especies del género Opisthonema. Iniversidad Autónoma de Baja California 2020-09-28 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article Artículo Arbitrado application/pdf text/xml text/xml https://www.cienciasmarinas.com.mx/index.php/cmarinas/article/view/3059 10.7773/cm.v46i3.3059 Ciencias Marinas; Vol. 46 No. 3 (2020); 145–154 Ciencias Marinas; Vol. 46 Núm. 3 (2020); 145–154 2395-9053 0185-3880 eng https://www.cienciasmarinas.com.mx/index.php/cmarinas/article/view/3059/420420503 https://www.cienciasmarinas.com.mx/index.php/cmarinas/article/view/3059/420420954 https://www.cienciasmarinas.com.mx/index.php/cmarinas/article/view/3059/420420659 Copyright (c) 2020 Ciencias Marinas https://creativecommons.org/licenses/by/4.0
institution Ciencias Marinas
collection OJS
language eng
format Online
author Pérez-Quiñonez, Carlos Iván
Quiñonez-Velázquez, Casimiro
García-Rodríguez, Francisco Javier
spellingShingle Pérez-Quiñonez, Carlos Iván
Quiñonez-Velázquez, Casimiro
García-Rodríguez, Francisco Javier
A simple method for the genetic identification of commercially important species in the Opisthonema genus Gill, 1861 in the southern Gulf of California
author_facet Pérez-Quiñonez, Carlos Iván
Quiñonez-Velázquez, Casimiro
García-Rodríguez, Francisco Javier
author_sort Pérez-Quiñonez, Carlos Iván
title A simple method for the genetic identification of commercially important species in the Opisthonema genus Gill, 1861 in the southern Gulf of California
title_short A simple method for the genetic identification of commercially important species in the Opisthonema genus Gill, 1861 in the southern Gulf of California
title_full A simple method for the genetic identification of commercially important species in the Opisthonema genus Gill, 1861 in the southern Gulf of California
title_fullStr A simple method for the genetic identification of commercially important species in the Opisthonema genus Gill, 1861 in the southern Gulf of California
title_full_unstemmed A simple method for the genetic identification of commercially important species in the Opisthonema genus Gill, 1861 in the southern Gulf of California
title_sort simple method for the genetic identification of commercially important species in the opisthonema genus gill, 1861 in the southern gulf of california
description The polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique was used to identify 3 species in the genus Opisthonema (Opisthonema bulleri, Opisthonema medirastre, and Opisthonema libertate) in the southern Gulf of California. Two specific primer pairs were designed to amplify the COI mitochondrial gene and 3 restriction enzymes (HpaII, RsaI, and AvaII) were selected to compare the RFLP patterns among the species. Different polymorphism levels were detected among samples. Easily analyzed specific restriction profiles were created, which differentiated unequivocally among the 3 target species. The approach undertaken here using PCR-RFLP is relatively cheap, fast, and robust, providing a useful technique for the identification of species in the genus Opisthonema.
publisher Iniversidad Autónoma de Baja California
publishDate 2020
url https://www.cienciasmarinas.com.mx/index.php/cmarinas/article/view/3059
_version_ 1792606981737938944

Ejemplares similares