Identification of pathogenic bacteria in fishes caught in the Pacific off Nicaragua

Identification of pathogenic bacteria in fishes caught in the Pacific off Nicaragua

The aim of this study was to identify pathogenic bacteria in fishes from 4 communities on the western Pacific coast of Nicaragua. A total of 62 fishes were collected for microbiological analysis using muscle samples, which were inoculated on thiosulfate-citrate-bile salts-sucrose agar and trypticase...

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Autores principales: Flores, Byron, González, Nelly, Bravo, Arlen, Mora-Sánchez, Brenda, Torres, Dayana, Jirón, William, Sheleby-Elías, Jessica, Balcázar, José L
Formato: Online
Lenguaje:eng
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Publicado: Iniversidad Autónoma de Baja California 2021
Acceso en línea:https://www.cienciasmarinas.com.mx/index.php/cmarinas/article/view/3212
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institution Ciencias Marinas
collection OJS
language eng
spa
format Online
author Flores, Byron
González, Nelly
Bravo, Arlen
Mora-Sánchez, Brenda
Torres, Dayana
Jirón, William
Sheleby-Elías, Jessica
Balcázar, José L
spellingShingle Flores, Byron
González, Nelly
Bravo, Arlen
Mora-Sánchez, Brenda
Torres, Dayana
Jirón, William
Sheleby-Elías, Jessica
Balcázar, José L
Identification of pathogenic bacteria in fishes caught in the Pacific off Nicaragua
author_facet Flores, Byron
González, Nelly
Bravo, Arlen
Mora-Sánchez, Brenda
Torres, Dayana
Jirón, William
Sheleby-Elías, Jessica
Balcázar, José L
author_sort Flores, Byron
title Identification of pathogenic bacteria in fishes caught in the Pacific off Nicaragua
title_short Identification of pathogenic bacteria in fishes caught in the Pacific off Nicaragua
title_full Identification of pathogenic bacteria in fishes caught in the Pacific off Nicaragua
title_fullStr Identification of pathogenic bacteria in fishes caught in the Pacific off Nicaragua
title_full_unstemmed Identification of pathogenic bacteria in fishes caught in the Pacific off Nicaragua
title_sort identification of pathogenic bacteria in fishes caught in the pacific off nicaragua
description The aim of this study was to identify pathogenic bacteria in fishes from 4 communities on the western Pacific coast of Nicaragua. A total of 62 fishes were collected for microbiological analysis using muscle samples, which were inoculated on thiosulfate-citrate-bile salts-sucrose agar and trypticase soy agar. Bacterial identification was carried out by biochemical tests and tests of tolerance to different NaCl concentrations, and the Kirby–Bauer method was used to determine the bacterial resistance. The results revealed the presence of Vibrio metschnikovii in 30.64% (95% CI: 18.36–42.92) of the fish samples, while Salinivibrio costicola was detected in 22.58% (95% CI: 11.37–33.79) of the samples. Coagulase-negative staphylococci (CoNS) were detected in 82.30% (95% CI: 71.94–92.57) of the samples. Species of the genus Vibrio were detected at a concentration of 3.9 × 103 CFU·g–1 in fish samples from the Las Peñitas community and 2.52 × 103 CFU·g–1 in samples from Poneloya, and these values were significantly higher (P = 0.003) than those found for samples from El Tránsito (5.25 × 102 CFU·g–1). Furthermore, 100% of the V. metschnikovii and S. costicola strains were resistant to amoxicillin/clavulanic acid and sensitive to ciprofloxacin. The bacterial load for S. costicola is typical of marine environments and does not represent a health hazard. However, V. metschnikovii can be present in raw fish and it therefore represents a public health hazard, as do CoNS, which are indicators of anthropogenic contamination.
publisher Iniversidad Autónoma de Baja California
publishDate 2021
url https://www.cienciasmarinas.com.mx/index.php/cmarinas/article/view/3212
_version_ 1792095474437586944
spelling oai:cienciasmarinas.com.mx:article-32122023-11-25T00:39:34Z Identification of pathogenic bacteria in fishes caught in the Pacific off Nicaragua Identificación de bacterias patógenas en peces capturados en el Pacífico frente a Nicaragua Flores, Byron González, Nelly Bravo, Arlen Mora-Sánchez, Brenda Torres, Dayana Jirón, William Sheleby-Elías, Jessica Balcázar, José L fish Vibrio Nicaragua Staphylococcus peces Vibrio Nicaragua Staphylococcus The aim of this study was to identify pathogenic bacteria in fishes from 4 communities on the western Pacific coast of Nicaragua. A total of 62 fishes were collected for microbiological analysis using muscle samples, which were inoculated on thiosulfate-citrate-bile salts-sucrose agar and trypticase soy agar. Bacterial identification was carried out by biochemical tests and tests of tolerance to different NaCl concentrations, and the Kirby–Bauer method was used to determine the bacterial resistance. The results revealed the presence of Vibrio metschnikovii in 30.64% (95% CI: 18.36–42.92) of the fish samples, while Salinivibrio costicola was detected in 22.58% (95% CI: 11.37–33.79) of the samples. Coagulase-negative staphylococci (CoNS) were detected in 82.30% (95% CI: 71.94–92.57) of the samples. Species of the genus Vibrio were detected at a concentration of 3.9 × 103 CFU·g–1 in fish samples from the Las Peñitas community and 2.52 × 103 CFU·g–1 in samples from Poneloya, and these values were significantly higher (P = 0.003) than those found for samples from El Tránsito (5.25 × 102 CFU·g–1). Furthermore, 100% of the V. metschnikovii and S. costicola strains were resistant to amoxicillin/clavulanic acid and sensitive to ciprofloxacin. The bacterial load for S. costicola is typical of marine environments and does not represent a health hazard. However, V. metschnikovii can be present in raw fish and it therefore represents a public health hazard, as do CoNS, which are indicators of anthropogenic contamination. El objetivo de este estudio fue identificar bacterias patógenas en peces procedentes de 4 comunidades en la costa del Pacífico en el occidente de Nicaragua. Se recolectaron 62 peces para realizar el análisis microbiológico a partir de muestras de músculo, las cuales fueron inoculadas en agar tiosulfato citrato bilis sacarosa y agar tripticasa de soja. La identificación bacteriana se realizó mediante pruebas bioquímicas y de tolerancia a diferentes concentraciones de NaCl, y para determinar la resistencia bacteriana, se empleó el método Kirby-Bauer. Los resultados mostraron presencia de Vibrio metschnikovii en un 30.64% (IC 95%: 18.36–42.92) de las muestras de peces, mientras que Salinivibrio costicola fue detectada en un 22.58% (IC 95%: 11.37–33.79) de las muestras. Staphylococcus coagulasa negativa (SCN) fue detectada en un 82.30% (IC 95%: 71.94–92.57) de las muestras. Especies del género Vibrio fueron detectadas a una concentración de 3.9 × 103 UFC·g–1 en las muestras de peces de la comunidad Las Peñitas y de 2.52 × 103 UFC·g–1 en las de Poneloya, y estos valores fueron significativamente mayores (P = 0.003) que aquellos encontrados para las muestras de El Tránsito (5.25 × 102 UFC·g–1). Además, el 100% de las cepas de V. metschnikovii y S. costicola fueron resistentes a amoxicilina/ácido clavulánico y sensibles a ciprofloxacina. La carga bacteriana de S. costicola es propia de ambientes marinos y no representa riesgo sanitario. Sin embargo, V. metschnikovii puede estar presente en el pescado crudo y, por lo tanto, representa un peligro para la salud pública, así como también lo es SCN, que es un indicador de contaminación antropogénica. Iniversidad Autónoma de Baja California 2021-09-29 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article Artículo Arbitrado application/pdf text/xml text/xml https://www.cienciasmarinas.com.mx/index.php/cmarinas/article/view/3212 10.7773/cm.v47i3.3212 Ciencias Marinas; Vol. 47 No. 3 (2021); 175–184 Ciencias Marinas; Vol. 47 Núm. 3 (2021); 175–184 2395-9053 0185-3880 eng spa https://www.cienciasmarinas.com.mx/index.php/cmarinas/article/view/3212/420420576 https://www.cienciasmarinas.com.mx/index.php/cmarinas/article/view/3212/420420825 https://www.cienciasmarinas.com.mx/index.php/cmarinas/article/view/3212/420420826 Copyright (c) 2021 Ciencias Marinas https://creativecommons.org/licenses/by/4.0

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