Accumulation of copper and histopathological alterations in the oyster Crassostrea angulata

Accumulation of copper and histopathological alterations in the oyster Crassostrea angulata

Specimens of Crassostrea angulata were exposed to sublethal copper concentrations (200 and 600 μgL–1 Cu2+) during 2 to 30 days. The accumulation of copper and histopathological effects on the gills, digestive gland and heart were studied. The highest copper concentrations were found in the gills, wi...

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Prif Awduron: Rodríguez de la Rua, A, Arellano, JM, González de Canales, ML, Blasco, J, Sarasquete, C
Fformat: Online
Iaith:eng
Cyhoeddwyd: Iniversidad Autónoma de Baja California 2005
Mynediad Ar-lein:https://www.cienciasmarinas.com.mx/index.php/cmarinas/article/view/39
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id oai:cienciasmarinas.com.mx:article-39
record_format ojs
institution Ciencias Marinas
collection OJS
language eng
format Online
author Rodríguez de la Rua, A
Arellano, JM
González de Canales, ML
Blasco, J
Sarasquete, C
spellingShingle Rodríguez de la Rua, A
Arellano, JM
González de Canales, ML
Blasco, J
Sarasquete, C
Accumulation of copper and histopathological alterations in the oyster Crassostrea angulata
author_facet Rodríguez de la Rua, A
Arellano, JM
González de Canales, ML
Blasco, J
Sarasquete, C
author_sort Rodríguez de la Rua, A
title Accumulation of copper and histopathological alterations in the oyster Crassostrea angulata
title_short Accumulation of copper and histopathological alterations in the oyster Crassostrea angulata
title_full Accumulation of copper and histopathological alterations in the oyster Crassostrea angulata
title_fullStr Accumulation of copper and histopathological alterations in the oyster Crassostrea angulata
title_full_unstemmed Accumulation of copper and histopathological alterations in the oyster Crassostrea angulata
title_sort accumulation of copper and histopathological alterations in the oyster crassostrea angulata
description Specimens of Crassostrea angulata were exposed to sublethal copper concentrations (200 and 600 μgL–1 Cu2+) during 2 to 30 days. The accumulation of copper and histopathological effects on the gills, digestive gland and heart were studied. The highest copper concentrations were found in the gills, with values over 2 mg g–1 dry weight for organisms exposed to the highest concentration at the end of the exposure period (day 30). In the digestive gland, the concentration was 1 mg g–1 dry weight (highest exposure on day 30). The rate of bioconcentration (BCr, defined as the concentration in the tissue at an exposure concentration at time t minus the mean concentration of the control throughout the experiment, divided by the exposure time) decreased in both tissues. The values ranged from 392 to 57 μg g–1 day–1 for gills and from 133 to 18 μg g–1 d–1 for the digestive gland. In the gills, specimens exposed to 200 μg L Cu2+ showed disorganization and apical alterations of the cilia cells and hyperplasia, lamellar fusion and lamellar loss in organisms exposed to 600 μg L–1 Cu2+. In the digestive gland, specimen exposed to 200 μg L–1 Cu2+ showed hemocytic infiltration in the underlying connective tissue and numerous brown cells compared to the control specimens. On the other hand, thinning of the epithelium of the digestive tubules, occlusion in the lumen of some primary tubules and dilation of the digestive ducts occurred in organisms exposed to 600 μg L–1 Cu2+. The heart of oyster exposed to copper showed thinning of the epithelium of the auricles and ventricle and an increase in brown cells on the walls of the auricles, as well as connective tissue destruction in the auricles and ventricle.
publisher Iniversidad Autónoma de Baja California
publishDate 2005
url https://www.cienciasmarinas.com.mx/index.php/cmarinas/article/view/39
_version_ 1715723927198629888
spelling oai:cienciasmarinas.com.mx:article-392019-04-09T22:51:11Z Accumulation of copper and histopathological alterations in the oyster Crassostrea angulata Acumulación de cobre y alteraciones histopatológicas en el ostión Crassostrea angulata Rodríguez de la Rua, A Arellano, JM González de Canales, ML Blasco, J Sarasquete, C accumulation histopathological copper Crassostrea angulata acumulación alteraciones hispatológicas cobre Crassostrea angulata Specimens of Crassostrea angulata were exposed to sublethal copper concentrations (200 and 600 μgL–1 Cu2+) during 2 to 30 days. The accumulation of copper and histopathological effects on the gills, digestive gland and heart were studied. The highest copper concentrations were found in the gills, with values over 2 mg g–1 dry weight for organisms exposed to the highest concentration at the end of the exposure period (day 30). In the digestive gland, the concentration was 1 mg g–1 dry weight (highest exposure on day 30). The rate of bioconcentration (BCr, defined as the concentration in the tissue at an exposure concentration at time t minus the mean concentration of the control throughout the experiment, divided by the exposure time) decreased in both tissues. The values ranged from 392 to 57 μg g–1 day–1 for gills and from 133 to 18 μg g–1 d–1 for the digestive gland. In the gills, specimens exposed to 200 μg L Cu2+ showed disorganization and apical alterations of the cilia cells and hyperplasia, lamellar fusion and lamellar loss in organisms exposed to 600 μg L–1 Cu2+. In the digestive gland, specimen exposed to 200 μg L–1 Cu2+ showed hemocytic infiltration in the underlying connective tissue and numerous brown cells compared to the control specimens. On the other hand, thinning of the epithelium of the digestive tubules, occlusion in the lumen of some primary tubules and dilation of the digestive ducts occurred in organisms exposed to 600 μg L–1 Cu2+. The heart of oyster exposed to copper showed thinning of the epithelium of the auricles and ventricle and an increase in brown cells on the walls of the auricles, as well as connective tissue destruction in the auricles and ventricle. Ejemplares de ostión Crassostrea angulata fueron expuestos a concentraciones subletales de cobre (200 y 600 μg L–1 Cu2+) durante un periodo de 2 a 30 días. Se cuantificó la concentración de cobre, así como las alteraciones histopatológicas inducidas en branquias, glándula digestiva y corazón. Las concentraciones más elevadas de cobre correspondieron a las branquias, con valores alrededor de 2 mg g–1 peso seco en los organismos expuestos a la concentración más alta, al final del periodo de exposición (día 30). En la glándula digestiva la concentración alcanzada fue del orden de 1 mg g–1 peso seco. La tasa de bioconcentración (BCr), definida como la diferencia entre la concentración en el tejido a una concentración de exposición a tiempo t y la concentración media del control a lo largo del experimento, dividida por el tiempo de exposición, disminuyó en ambos tejidos. Los valores variaron en el intervalo entre 392 y 57 μg g–1 d–1 en las branquias y entre 133 y 18 μg g–1 día–1 en la glándula digestiva. En branquias de ejemplares tratados con una concentración de 200 μgL–1Cu2+ se observó una desorganización del tejido conjuntivo, alteraciones en la porción apical de las células ciliadas e hiperplasia y fusión de laminillas, pudiendo llegar incluso a la pérdida de estas laminillas a 600 μgL–1Cu2+. En la glándula digestiva (hepatopáncreas) de ejemplares sometidos a 600 μgL–1Cu2+ se detectó un adelgazamiento del epitelio y, en algunos casos, oclusión de la luz de los túbulos digestivos y dilatación de los conductos digestivos. En el corazón de los organismos expuestos a concentraciones subletales de cobre se observó un adelgazamiento del epitelio de las aurículas y del ventrículo, un incremento de las células marrones (brown cells) en las paredes de las aurículas, así como una distensión de las fibras musculares y destrucción del tejido conectivo de soporte, tanto en las aurículas como en el ventrículo. Iniversidad Autónoma de Baja California 2005-03-06 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article Artículo Arbitrado application/pdf https://www.cienciasmarinas.com.mx/index.php/cmarinas/article/view/39 10.7773/cm.v31i3.39 Ciencias Marinas; Vol. 31 No. 3 (2005); 455-466 Ciencias Marinas; Vol. 31 Núm. 3 (2005); 455-466 2395-9053 0185-3880 eng https://www.cienciasmarinas.com.mx/index.php/cmarinas/article/view/39/21

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