Pigment variations induced by the oxygen level of the medium in a marine bacterium alteromonas sp. (strain cect 4800)

Pigment variations induced by the oxygen level of the medium in a marine bacterium alteromonas sp. (strain cect 4800)

A red-pigmented bacterial strain was isolated from indoor cultures of the marine microalga Tetraselmis suecica Butcher (Prasynophyceae) and identified as belonging to the genus Alteromonas (strain CECT 4800). Pigment composition was analyzed by means of high-performance liquid chromatography (HPLC),...

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Autores principales: González-Valle, MA, Montero, O, Moreno-Garrido, I, Lubian, LM
Formato: Online
Idioma:eng
Publicado em: Iniversidad Autónoma de Baja California 2000
Acesso em linha:https://www.cienciasmarinas.com.mx/index.php/cmarinas/article/view/570
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id oai:cienciasmarinas.com.mx:article-570
record_format ojs
institution Ciencias Marinas
collection OJS
language eng
format Online
author González-Valle, MA
Montero, O
Moreno-Garrido, I
Lubian, LM
spellingShingle González-Valle, MA
Montero, O
Moreno-Garrido, I
Lubian, LM
Pigment variations induced by the oxygen level of the medium in a marine bacterium alteromonas sp. (strain cect 4800)
author_facet González-Valle, MA
Montero, O
Moreno-Garrido, I
Lubian, LM
author_sort González-Valle, MA
title Pigment variations induced by the oxygen level of the medium in a marine bacterium alteromonas sp. (strain cect 4800)
title_short Pigment variations induced by the oxygen level of the medium in a marine bacterium alteromonas sp. (strain cect 4800)
title_full Pigment variations induced by the oxygen level of the medium in a marine bacterium alteromonas sp. (strain cect 4800)
title_fullStr Pigment variations induced by the oxygen level of the medium in a marine bacterium alteromonas sp. (strain cect 4800)
title_full_unstemmed Pigment variations induced by the oxygen level of the medium in a marine bacterium alteromonas sp. (strain cect 4800)
title_sort pigment variations induced by the oxygen level of the medium in a marine bacterium alteromonas sp. (strain cect 4800)
description A red-pigmented bacterial strain was isolated from indoor cultures of the marine microalga Tetraselmis suecica Butcher (Prasynophyceae) and identified as belonging to the genus Alteromonas (strain CECT 4800). Pigment composition was analyzed by means of high-performance liquid chromatography (HPLC), and spectral characteristics of three major pigments with reddish and yellowish colorations were determined. Changes in pigment composition during growth in liquid medium containing either ferric citrate, sodium citrate, glucose or glucose plus iron, as well as in relation to the oxygen concentration in the liquid medium were investigated. Pigment production was found to be enhanced with culture aging, the highest enhancement occurring in iron-deprived cultures. An increase in the major pigment (a red pigment) content of 70% in relation to the initial one took place when a culture fed with ferric citrate was bubbled with molecular oxygen. In contrast, red pigment content decreased to about 50% of the initial content when the culture was sparged with nitrogen. Other pigments did not exhibit substantial modifications. Increase in the red pigment content was also observed after the different cultures were exposed to diverse oxygen concentrations for one hour. Similar results were obtained in cultures submitted to both strong aeration and agitation (5 mL min–1 air and 500 rpm, respectively). Findings of this study suggest that pigment production by Alteromonas sp. (strain CECT 4800), in particular that of the red pigment, could rely on the oxygen concentration in the culture medium due to antioxidative reactions.
publisher Iniversidad Autónoma de Baja California
publishDate 2000
url https://www.cienciasmarinas.com.mx/index.php/cmarinas/article/view/570
_version_ 1715723984558882816
spelling oai:cienciasmarinas.com.mx:article-5702019-05-09T22:42:04Z Pigment variations induced by the oxygen level of the medium in a marine bacterium alteromonas sp. (strain cect 4800) Variaciones inducidas por el nivel de oxígeno del medio en los pigmentos de la bacteria marina Alteromonas sp.(CEPA CECT 4800) González-Valle, MA Montero, O Moreno-Garrido, I Lubian, LM bacterias marinas Alteromonas sp xígeno molecular pigmento HPLC marine bacteria Alteromonas sp molecular oxygen pigment HPLC A red-pigmented bacterial strain was isolated from indoor cultures of the marine microalga Tetraselmis suecica Butcher (Prasynophyceae) and identified as belonging to the genus Alteromonas (strain CECT 4800). Pigment composition was analyzed by means of high-performance liquid chromatography (HPLC), and spectral characteristics of three major pigments with reddish and yellowish colorations were determined. Changes in pigment composition during growth in liquid medium containing either ferric citrate, sodium citrate, glucose or glucose plus iron, as well as in relation to the oxygen concentration in the liquid medium were investigated. Pigment production was found to be enhanced with culture aging, the highest enhancement occurring in iron-deprived cultures. An increase in the major pigment (a red pigment) content of 70% in relation to the initial one took place when a culture fed with ferric citrate was bubbled with molecular oxygen. In contrast, red pigment content decreased to about 50% of the initial content when the culture was sparged with nitrogen. Other pigments did not exhibit substantial modifications. Increase in the red pigment content was also observed after the different cultures were exposed to diverse oxygen concentrations for one hour. Similar results were obtained in cultures submitted to both strong aeration and agitation (5 mL min–1 air and 500 rpm, respectively). Findings of this study suggest that pigment production by Alteromonas sp. (strain CECT 4800), in particular that of the red pigment, could rely on the oxygen concentration in the culture medium due to antioxidative reactions. Se ha aislado una cepa bacteriana de color rojo a partir de cultivos de laboratorio de la microalga marina Tetraselmis suecica Butcher (Prasynophyceae), siendo identificada taxonómicamente como perteneciente al género Alteromonas (cepa CECT 4800). Su composición pigmentaria fue analizada mediante cromatografía líquida de alta resolución (HPLC), y se determinaron las características espectrales de sus tres pigmentos mayoritarios, que presentaban coloraciones roja y amarilla. Asimismo, se investigaron los cambios pigmentarios durante el crecimiento en medio líquido que contenía ya sea citrato sódico, citrato férrico, glucosa o glucosa más hierro, así como en relación con la concentración de oxígeno disuelto en el medio líquido. Se observó que la producción de pigmento se incrementaba con la edad del cultivo, siendo las producciones más altas en cultivos carentes de hierro. Se detectó un incremento del 70% en el contenido del pigmento mayoritario (de color rojo) en relación con el contenido inicial cuando un cultivo suplementado con citrato férrico se burbujeaba con oxígeno molecular. Por el contrario, la concentración de pigmento rojo sufrió un descenso del 50% con relación a la concentración original cuando el cultivo fue burbujeado con nitrógeno. El resto de los pigmentos no mostraron variaciones substanciales. Asimismo, se produjo un incremento de la concentración del pigmento rojo tras la exposición de distintos cultivos a diversas concentraciones de oxígeno durante una hora. Resultados similares se obtuvieron en cultivos sometidos a aireación fuerte y agitación (5 mL min–1 y 500 rpm, respectivamente). Los resultados de este estudio sugieren que la producción de pigmentos por parte de Alteromonas sp. (cepa CECT 4800), en particular la producción de pigmento rojo, podría estar relacionado con la cantidad de oxígeno disuelto debido a reacciones antioxidativas. Iniversidad Autónoma de Baja California 2000-03-06 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article Artículo Arbitrado application/pdf https://www.cienciasmarinas.com.mx/index.php/cmarinas/article/view/570 10.7773/cm.v26i1.570 Ciencias Marinas; Vol. 26 No. 1 (2000); 77-96 Ciencias Marinas; Vol. 26 Núm. 1 (2000); 77-96 2395-9053 0185-3880 eng https://www.cienciasmarinas.com.mx/index.php/cmarinas/article/view/570/507

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