A cytogenetic method to obtain chromosomes for studies of banding and fine morphology of karyotypes in bivalve mollusks of the family Ostreidae

A cytogenetic method to obtain chromosomes for studies of banding and fine morphology of karyotypes in bivalve mollusks of the family Ostreidae

The studies of banding patterns of chromosomes in bivalve mollusks of the family Ostreidae have not been developed satisfactorily due to the lack of a suitable method that enables the obtention of chromosomes in a proper condition. The production of chromosomal fields in cold by means of a modificat...

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Autores principales: Rodríguez-Romero, F, Gasca-Montes-de-Oca, M, De-la-Rosa-Vélez, J
Formato: Online
Lenguaje:eng
Publicado: Iniversidad Autónoma de Baja California 1991
Acceso en línea:https://www.cienciasmarinas.com.mx/index.php/cmarinas/article/view/850
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spelling oai:cienciasmarinas.com.mx:article-8502020-06-06T00:58:57Z A cytogenetic method to obtain chromosomes for studies of banding and fine morphology of karyotypes in bivalve mollusks of the family Ostreidae Un método citogenético para la obtención de cromosomas para estudios de bandeo y morfología fina de los cariotipos de moluscos bivalvos de la familia ostreidae Rodríguez-Romero, F Gasca-Montes-de-Oca, M De-la-Rosa-Vélez, J n/a n/a The studies of banding patterns of chromosomes in bivalve mollusks of the family Ostreidae have not been developed satisfactorily due to the lack of a suitable method that enables the obtention of chromosomes in a proper condition. The production of chromosomal fields in cold by means of a modification to the dripping and air drying method from embryos and larvae obtained from in vitro fertilization, and the control of the spiralization degree of the chromosomes, are achieved by the application of colchicine pulses for periods of 30, 45 and 60 minutes. These chromosomes show a certain degree of synchronisation due to the use of oyster early embryos and larvae in which the cell division is very frequent. Separation of cells in division was achieved with a 50% acetic acid solution, at 37ºC. Chromosomes obtained in this manner can be stained successfully for the severa1 different bands willing to be studied. Los estudios de los patrones de bandeo en los cromosomas de moluscos bivalvos de la familia Ostreidae no han sido desarrollados lo suficiente debido a la carencia de un método apropiado que permita la obtención de cromosomas en condiciones adecuadas para su realización. La producción de campos cromosómicos en frío mediante una modificación al procedimiento de goteo y secado al aire a partir de embriones y larvas obtenidos por fertilización in vitro y el control del grado de espiralización de los cromosomas se logra por la aplicación de pulsos de colchicina por periodos de 30, 45 y 60 minutos. Estos cromosomas presentan cierto grado de sincronización debido al uso de embriones tempranos y larvas de ostiones en los cuales las divisiones celulares son muy frecuentes. La separación de las células en división fue lograda con una solución de ácido acético al 50% a 37ºC. Los cromosomas así obtenidos pueden ser teñidos con éxito para los distintos tipos de bandas que se deseen estudiar. Iniversidad Autónoma de Baja California 1991-03-06 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article Artículo Arbitrado application/pdf https://www.cienciasmarinas.com.mx/index.php/cmarinas/article/view/850 10.7773/cm.v17i4.850 Ciencias Marinas; Vol. 17 No. 4 (1991); 1-10 Ciencias Marinas; Vol. 17 Núm. 4 (1991); 1-10 2395-9053 0185-3880 eng https://www.cienciasmarinas.com.mx/index.php/cmarinas/article/view/850/774
institution Ciencias Marinas
collection OJS
language eng
format Online
author Rodríguez-Romero, F
Gasca-Montes-de-Oca, M
De-la-Rosa-Vélez, J
spellingShingle Rodríguez-Romero, F
Gasca-Montes-de-Oca, M
De-la-Rosa-Vélez, J
A cytogenetic method to obtain chromosomes for studies of banding and fine morphology of karyotypes in bivalve mollusks of the family Ostreidae
author_facet Rodríguez-Romero, F
Gasca-Montes-de-Oca, M
De-la-Rosa-Vélez, J
author_sort Rodríguez-Romero, F
title A cytogenetic method to obtain chromosomes for studies of banding and fine morphology of karyotypes in bivalve mollusks of the family Ostreidae
title_short A cytogenetic method to obtain chromosomes for studies of banding and fine morphology of karyotypes in bivalve mollusks of the family Ostreidae
title_full A cytogenetic method to obtain chromosomes for studies of banding and fine morphology of karyotypes in bivalve mollusks of the family Ostreidae
title_fullStr A cytogenetic method to obtain chromosomes for studies of banding and fine morphology of karyotypes in bivalve mollusks of the family Ostreidae
title_full_unstemmed A cytogenetic method to obtain chromosomes for studies of banding and fine morphology of karyotypes in bivalve mollusks of the family Ostreidae
title_sort cytogenetic method to obtain chromosomes for studies of banding and fine morphology of karyotypes in bivalve mollusks of the family ostreidae
description The studies of banding patterns of chromosomes in bivalve mollusks of the family Ostreidae have not been developed satisfactorily due to the lack of a suitable method that enables the obtention of chromosomes in a proper condition. The production of chromosomal fields in cold by means of a modification to the dripping and air drying method from embryos and larvae obtained from in vitro fertilization, and the control of the spiralization degree of the chromosomes, are achieved by the application of colchicine pulses for periods of 30, 45 and 60 minutes. These chromosomes show a certain degree of synchronisation due to the use of oyster early embryos and larvae in which the cell division is very frequent. Separation of cells in division was achieved with a 50% acetic acid solution, at 37ºC. Chromosomes obtained in this manner can be stained successfully for the severa1 different bands willing to be studied.
publisher Iniversidad Autónoma de Baja California
publishDate 1991
url https://www.cienciasmarinas.com.mx/index.php/cmarinas/article/view/850
_version_ 1715724019146162176

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