Automation of inoculation in culture media for the microbiology laboratory

Automation of inoculation in culture media for the microbiology laboratory

The clinical laboratories belonging to the health system in Mexico are among the most discriminated departments due to factors such as sources of financing, as it requires strong administrative management, as well as the dependence on vast infrastructure, material, and human resources. Pneumonia, a...

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Autores principales: Flores Medina, Pablo Jonatán, Garibay Murillo, Paola, Peñaloza Mendoza, Guillermo Rey
Formato: Online
Lenguaje:spa
Publicado: Universidad Autónoma de Baja California 2023
Acceso en línea:https://recit.uabc.mx/index.php/revista/article/view/285
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id recit-article-285
record_format ojs
institution RECIT
collection OJS
language spa
format Online
author Flores Medina, Pablo Jonatán
Garibay Murillo, Paola
Peñaloza Mendoza, Guillermo Rey
spellingShingle Flores Medina, Pablo Jonatán
Garibay Murillo, Paola
Peñaloza Mendoza, Guillermo Rey
Automation of inoculation in culture media for the microbiology laboratory
author_facet Flores Medina, Pablo Jonatán
Garibay Murillo, Paola
Peñaloza Mendoza, Guillermo Rey
author_sort Flores Medina, Pablo Jonatán
title Automation of inoculation in culture media for the microbiology laboratory
title_short Automation of inoculation in culture media for the microbiology laboratory
title_full Automation of inoculation in culture media for the microbiology laboratory
title_fullStr Automation of inoculation in culture media for the microbiology laboratory
title_full_unstemmed Automation of inoculation in culture media for the microbiology laboratory
title_sort automation of inoculation in culture media for the microbiology laboratory
description The clinical laboratories belonging to the health system in Mexico are among the most discriminated departments due to factors such as sources of financing, as it requires strong administrative management, as well as the dependence on vast infrastructure, material, and human resources. Pneumonia, a disease that can be detected in a subclinical stage, is the 2nd cause of death from respiratory infections in Mexico and is, in turn, the 7th cause of death in the country. For the early diagnosis of diseases caused by microorganisms such as the one just mentioned, laboratory tests are performed, including inoculation in culture media for the proliferation of said microorganisms, however, the techniques used are manual, delaying technological progress. applied in terms of automation, which would allow better workflows with fewer resources. A great challenge in automating the process is cross-contamination that occurs at the time of inoculation, as well as decontamination and sterilization after sowing. Considering this, the model of a device that performs the microbiological culture process automatically was developed, emphasizing the variables involved to avoid cross-contamination and sterilization of the areas, comparing various decontamination techniques, the ones that best fit us are through exposure to UV light, since we avoid the malfunction of electronic components due to temperature.
publisher Universidad Autónoma de Baja California
publishDate 2023
url https://recit.uabc.mx/index.php/revista/article/view/285
_version_ 1792095384552603648
spelling recit-article-2852024-02-13T02:55:52Z Automation of inoculation in culture media for the microbiology laboratory Automatización de inoculación en medios de cultivo para el laboratorio de microbiología Flores Medina, Pablo Jonatán Garibay Murillo, Paola Peñaloza Mendoza, Guillermo Rey Clinical laboratory Streak Culture sowing Microorganisms Cross contamination Laboratorio clínico Estría Siembra de cultivos Microorganismos Contaminación cruzada The clinical laboratories belonging to the health system in Mexico are among the most discriminated departments due to factors such as sources of financing, as it requires strong administrative management, as well as the dependence on vast infrastructure, material, and human resources. Pneumonia, a disease that can be detected in a subclinical stage, is the 2nd cause of death from respiratory infections in Mexico and is, in turn, the 7th cause of death in the country. For the early diagnosis of diseases caused by microorganisms such as the one just mentioned, laboratory tests are performed, including inoculation in culture media for the proliferation of said microorganisms, however, the techniques used are manual, delaying technological progress. applied in terms of automation, which would allow better workflows with fewer resources. A great challenge in automating the process is cross-contamination that occurs at the time of inoculation, as well as decontamination and sterilization after sowing. Considering this, the model of a device that performs the microbiological culture process automatically was developed, emphasizing the variables involved to avoid cross-contamination and sterilization of the areas, comparing various decontamination techniques, the ones that best fit us are through exposure to UV light, since we avoid the malfunction of electronic components due to temperature. Los laboratorios clínicos pertenecientes al sistema de salud en México son de los departamentos más discriminados debido a factores como fuentes de financiamiento ya que necesita de una fuerte gestión administrativa, así como la dependencia de basta infraestructura, recurso material y humano. La neumonía, enfermedad que puede detectarse en una etapa subclínica, es la 2da causa de muertes por infecciones respiratorias en México y está a su vez, es la 7ma causa de muerte en el país. Para el diagnóstico temprano de enfermedades causadas por microorganismos como la recién mencionada, se realizan exámenes de laboratorio, entre los que destacan la inoculación en medios de cultivo para la proliferación de dichos microorganismos, sin embargo, las técnicas utilizadas son manuales, retrasando el avance tecnológico aplicado en términos de automatización, lo que permitiría tener mejores flujos de trabajo con menos recursos. Un gran reto en la automatización del proceso es la contaminación cruzada que se da en el momento de realizar la inoculación, así como la descontaminación y esterilización posterior a la siembra. Considerando esto, se desarrolló el modelo de un dispositivo que realice el proceso de cultivos microbiológicos de forma automática, haciendo énfasis en las variables involucradas para evitar contaminación cruzada y la esterilización de las zonas, comparando diversas técnicas de descontaminación, las que mejor nos ajusta es por medio de exposición a luz UV, ya que evitamos el mal funcionamiento de componentes electrónicos por temperatura. Universidad Autónoma de Baja California 2023-11-09 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf text/html application/zip https://recit.uabc.mx/index.php/revista/article/view/285 10.37636/recit.v6n4e285 REVISTA DE CIENCIAS TECNOLÓGICAS; Vol. 6 No. 4 (2023): October-December; e285 REVISTA DE CIENCIAS TECNOLÓGICAS; Vol. 6 Núm. 4 (2023): Octubre-Diciembre; e285 2594-1925 spa https://recit.uabc.mx/index.php/revista/article/view/285/509 https://recit.uabc.mx/index.php/revista/article/view/285/510 https://recit.uabc.mx/index.php/revista/article/view/285/511 Copyright (c) 2023 Pablo Jonatán Flores Medina, Paola Garibay Murillo, Guillermo Rey Peñaloza Mendoza https://creativecommons.org/licenses/by/4.0

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