Micronuclei in reticulocytes of Balb / c mice orally treated with silver nanoparticles
The purpose of this work was to evaluate the genotoxic capacity of AgNPs Argovit™ with the micronucleus technique in mouse peripheral blood reticulocytes. Argovit™ is administered orally in therapeutic doses (indicated for dogs as treatment for distemper) for three days to an experimental group with...
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Universidad Autónoma de Baja California
2018
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repositorioinstitucional-20.500.12930-70072023-05-09T14:35:08Z Micronuclei in reticulocytes of Balb / c mice orally treated with silver nanoparticles Micronúcleos en reticulocitos de ratones Balb/c tratados de forma oral con nanopartículas de plata Castañeda-Yslas, Idalia Yazmin Ruiz-Ruiz, Balam Arellano-García, María Evarista Juárez-Moreno, Karla Oyuki Torres-Bugarín, Olivia Bogdanchikova, Nina Pestryakov, Alexey Radilla-Chávez, Patricia Silver Nanoparticles Micronuclei Reticulocytes Genotoxicity. Nanopartículas de Plata Micronúcleos Reticulocitos Genotoxicidad. The purpose of this work was to evaluate the genotoxic capacity of AgNPs Argovit™ with the micronucleus technique in mouse peripheral blood reticulocytes. Argovit™ is administered orally in therapeutic doses (indicated for dogs as treatment for distemper) for three days to an experimental group with seven mice. Another two groups with an equal number of mice were administered with injectable water and arabinose C, respectively. The specimens were measured and weighed before and after the experiment. Peripheral blood samples were also taken daily by a cut at the tip of the tail and then extended on slides for micronucleus test in reticulocytes. The smears were fixed in ethanol at 80 % and stained with acridine orange, after that, the presence of micronuclei was identified in 1000 reticulocytes counted by fluorescence microscopy. Preliminary results show significant differences in the number of micronuclei in reticulocytes between the three groups, so it’s necessary to compare this result with another type of in vitro models. El estudio de la genotoxicidad en modelos murinos es útil para responder las dudas relacionadas con la seguridad por el uso de nanomateriales, sobre todo cuando se pretende el desarrollo de nuevas opciones terapéuticas. El propósito de este trabajo fue evaluar la capacidad genotóxica de AgNPs Argovit™ a partir de la técnica de micronúcleos en reticulocitos de sangre periférica de ratones, prueba ampliamente utilizada para conocer el potencial genotóxico de diferentes agentes. Las nanopartículas de plata se suministraron en dosis terapéuticas por vía oral (indicadas para perros como tratamiento para el moquillo) durante tres días a un grupo conformado por siete ratones y a otros dos grupos con igual número de ratones se administró respectivamente agua inyectable y arabinosa C. Los especímenes fueron medidos, pesados antes y después del experimento. También se tomaron diariamente muestras de sangre periférica mediante un corte en la punta de la cola para posteriormente realizar extendidos sobre portaobjetos para la prueba de micronúcleos en reticulocitos. Los frotis se fijaron en etanol al 80 % y se tiñeron con anaranjado de acridina para posteriormente leer en 1000 reticulocitos la presencia de micronúcleos con microscopio de fluorescencia. Los resultados preliminares muestran diferencias significativas en el número de micronúcleos en reticulocitos entre los tres grupos, por lo que se requiere comprobar este resultado con otro tipo de modelos in vitro. 2018-01-01 2021-06-03T03:52:31Z 2021-06-03T03:52:31Z info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Research article Articulo de Investigación https://recit.uabc.mx/index.php/revista/article/view/oba14 10.37636/recit.v126568 https://hdl.handle.net/20.500.12930/7007 spa https://recit.uabc.mx/index.php/revista/article/view/oba14/93 https://recit.uabc.mx/index.php/revista/article/view/oba14/html https://recit.uabc.mx/index.php/revista/article/view/oba14/OBA14 Copyright (c) 2018 Castañeda-Yslas Idalia Yazmin, Ruiz-Ruiz Balam, Arellano-García María Evarista, Juárez-Moreno Karla Oyuki, Torres-Bugarín Olivia, Bogdanchikova Nina, Pestryakov Alexey, Radilla-Chávez Patricia http://creativecommons.org/licenses/by/4.0 application/pdf text/html application/xml Universidad Autónoma de Baja California REVISTA DE CIENCIAS TECNOLÓGICAS; Vol. 1 No. 2 (2018); 65-68 REVISTA DE CIENCIAS TECNOLÓGICAS; Vol. 1 Núm. 2 (2018); 65-68 2594-1925 |
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Silver Nanoparticles Micronuclei Reticulocytes Genotoxicity. Nanopartículas de Plata Micronúcleos Reticulocitos Genotoxicidad. |
| spellingShingle |
Silver Nanoparticles Micronuclei Reticulocytes Genotoxicity. Nanopartículas de Plata Micronúcleos Reticulocitos Genotoxicidad. Castañeda-Yslas, Idalia Yazmin Ruiz-Ruiz, Balam Arellano-García, María Evarista Juárez-Moreno, Karla Oyuki Torres-Bugarín, Olivia Bogdanchikova, Nina Pestryakov, Alexey Radilla-Chávez, Patricia Micronuclei in reticulocytes of Balb / c mice orally treated with silver nanoparticles |
| description |
The purpose of this work was to evaluate the genotoxic capacity of AgNPs Argovit™ with the micronucleus technique in mouse peripheral blood reticulocytes. Argovit™ is administered orally in therapeutic doses (indicated for dogs as treatment for distemper) for three days to an experimental group with seven mice. Another two groups with an equal number of mice were administered with injectable water and arabinose C, respectively. The specimens were measured and weighed before and after the experiment. Peripheral blood samples were also taken daily by a cut at the tip of the tail and then extended on slides for micronucleus test in reticulocytes. The smears were fixed in ethanol at 80 % and stained with acridine orange, after that, the presence of micronuclei was identified in 1000 reticulocytes counted by fluorescence microscopy. Preliminary results show significant differences in the number of micronuclei in reticulocytes between the three groups, so it’s necessary to compare this result with another type of in vitro models. |
| format |
info:eu-repo/semantics/article |
| author |
Castañeda-Yslas, Idalia Yazmin Ruiz-Ruiz, Balam Arellano-García, María Evarista Juárez-Moreno, Karla Oyuki Torres-Bugarín, Olivia Bogdanchikova, Nina Pestryakov, Alexey Radilla-Chávez, Patricia |
| author_facet |
Castañeda-Yslas, Idalia Yazmin Ruiz-Ruiz, Balam Arellano-García, María Evarista Juárez-Moreno, Karla Oyuki Torres-Bugarín, Olivia Bogdanchikova, Nina Pestryakov, Alexey Radilla-Chávez, Patricia |
| author_sort |
Castañeda-Yslas, Idalia Yazmin |
| title |
Micronuclei in reticulocytes of Balb / c mice orally treated with silver nanoparticles |
| title_short |
Micronuclei in reticulocytes of Balb / c mice orally treated with silver nanoparticles |
| title_full |
Micronuclei in reticulocytes of Balb / c mice orally treated with silver nanoparticles |
| title_fullStr |
Micronuclei in reticulocytes of Balb / c mice orally treated with silver nanoparticles |
| title_full_unstemmed |
Micronuclei in reticulocytes of Balb / c mice orally treated with silver nanoparticles |
| title_sort |
micronuclei in reticulocytes of balb / c mice orally treated with silver nanoparticles |
| publisher |
Universidad Autónoma de Baja California |
| publishDate |
2018 |
| url |
https://recit.uabc.mx/index.php/revista/article/view/oba14 |
| _version_ |
1792609716622327808 |
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