Immunological responses and wound healing in the polychaete Eurythoe complanata (Annelida: Amphinomidae) exposed to copper

Polychaetes exposed to 0.4 mg Cu+2 L–1 (2.14 mg CuSO4 5H2O L–1; 30% 96 h-LC50) for seven days and control individuals were divided into three groups: the first two were sensitized and non-sensitized with Micrococcus lysodeikticus, and the third was sensitized with heat-killed yeast. Lysozyme activit...

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Autores principales:	Zapata-Vívenes, E, Nusetti, OA, Marcano, L, Esclapés, MM, Arredondo, L
Formato:	info:eu-repo/semantics/article
Lenguaje:	eng
Publicado:	Iniversidad Autónoma de Baja California 2005
Materias:	copper wound healing phagocytosis lysozyme Eurythoe complanata cobre cicatrización fagocitosis lisozima Eurythoe companata
Acceso en línea:	https://www.cienciasmarinas.com.mx/index.php/cmarinas/article/view/83
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id	repositorioinstitucional-20.500.12930-7326
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spelling	repositorioinstitucional-20.500.12930-73262023-05-09T14:30:26Z Immunological responses and wound healing in the polychaete Eurythoe complanata (Annelida: Amphinomidae) exposed to copper Respuestas inmunológicas y cicatrización en el poliqueto Eurythoe complanata (Annelida: Amphinomid ae) expuesto a cobre Zapata-Vívenes, E Nusetti, OA Marcano, L Esclapés, MM Arredondo, L copper wound healing phagocytosis lysozyme Eurythoe complanata cobre cicatrización fagocitosis lisozima Eurythoe companata Polychaetes exposed to 0.4 mg Cu+2 L–1 (2.14 mg CuSO4 5H2O L–1; 30% 96 h-LC50) for seven days and control individuals were divided into three groups: the first two were sensitized and non-sensitized with Micrococcus lysodeikticus, and the third was sensitized with heat-killed yeast. Lysozyme activity was tested in the first two groups and phagocytosis in the third one. Lysozyme was measured at 2 h and phagocytosis 15 min after immune sensitization. The effect of copper on wound healing was also examined in the polychaetes. For this, the polychaetes exposed and not exposed to copper were cut in two halves, and the body fragments were held in aquaria containing clean seawater, and the cicatrization was periodically observed. The lysozyme and phagocytosis responses were induced by the sensitization; however, these responses were inhibited after the metal exposure. On the other hand, 60–80% of the body fragments from the control group healed within four days, whereas the copper-treated individuals did not show cicatrization in the same period of time. In conclusion, the inhibition of the experimental activation of lysozyme and phagocytosis, and of wound healing in the polychaete Eurythoe complanata exposed to acute copper exposure, suggests the metal´  s potential toxicity on the physiological mechanisms that modulate the innate immunity in annelids, whose alteration could affect their tolerance to microbial infection. Se dividieron grupos de poliquetos, controles y expuestos a 0.4 mg Cu+2 L–1 (2.14 mg CuSO4 5H2O L–1; 30% 96 h-CL50) por siete días, en tres grupos: sensibilizados y no sensibilizados con Micrococcus lysodeikticus para evaluar la actividad de lisozima del fluido celómico, y otro sensibilizado con levaduras inactivadas por calor para evaluar la respuesta fagocítica de los celomocitos. La lisozima fue medida a 2 h y la fagocitosis a los 15 min después de la sensibilización inmunológica. Además, se evaluó el efecto de la exposición al cobre sobre la respuesta de cicatrización en los poliquetos. Para ello, los organismos expuestos al cobre y no expuestos fueron cortados en dos mitades y mantenidos en acuarios conteniendo agua de mar (sin cobre), determinándose el porcentaje de individuos cicatrizados diariamente. Las respuestas de fagocitosis y lisozima fueron inducidas por la sensibilización e inhibidas después de la exposición al metal. Por otra parte, 60–80% de los individuos controles cicatrizaron al cabo de cuatro días, mientras que los individuos tratados con cobre no evidenciaron cicatrización en el mismo periodo de tiempo. En conclusión, la inhibición de la inducción experimental de la lisozima y la fagocitosis, y de la cicatrización en el poliqueto Eurythoe complanata por la exposición aguda al cobre, sugiere el potencial de toxicidad del metal sobre los mecanismos fisiológicos que modulan la inmunidad innata y la reparación de heridas en anélidos, funciones biológicas comunes en animales cuya supresión puede resultar en una disminuida tolerancia a infecciones microbianas. 2005-03-06 2021-06-03T03:54:55Z 2021-06-03T03:54:55Z info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article Artículo Arbitrado https://www.cienciasmarinas.com.mx/index.php/cmarinas/article/view/83 10.7773/cm.v31i11.83 https://hdl.handle.net/20.500.12930/7326 eng https://www.cienciasmarinas.com.mx/index.php/cmarinas/article/view/83/63 application/pdf Iniversidad Autónoma de Baja California Ciencias Marinas; Vol. 31 No. 1A (2005); 1-10 Ciencias Marinas; Vol. 31 Núm. 1A (2005); 1-10 2395-9053 0185-3880
institution	Repositorio Institucional
collection	DSpace
language	eng
topic	copper wound healing phagocytosis lysozyme Eurythoe complanata cobre cicatrización fagocitosis lisozima Eurythoe companata
spellingShingle	copper wound healing phagocytosis lysozyme Eurythoe complanata cobre cicatrización fagocitosis lisozima Eurythoe companata Zapata-Vívenes, E Nusetti, OA Marcano, L Esclapés, MM Arredondo, L Immunological responses and wound healing in the polychaete Eurythoe complanata (Annelida: Amphinomidae) exposed to copper
description	Polychaetes exposed to 0.4 mg Cu+2 L–1 (2.14 mg CuSO4 5H2O L–1; 30% 96 h-LC50) for seven days and control individuals were divided into three groups: the first two were sensitized and non-sensitized with Micrococcus lysodeikticus, and the third was sensitized with heat-killed yeast. Lysozyme activity was tested in the first two groups and phagocytosis in the third one. Lysozyme was measured at 2 h and phagocytosis 15 min after immune sensitization. The effect of copper on wound healing was also examined in the polychaetes. For this, the polychaetes exposed and not exposed to copper were cut in two halves, and the body fragments were held in aquaria containing clean seawater, and the cicatrization was periodically observed. The lysozyme and phagocytosis responses were induced by the sensitization; however, these responses were inhibited after the metal exposure. On the other hand, 60–80% of the body fragments from the control group healed within four days, whereas the copper-treated individuals did not show cicatrization in the same period of time. In conclusion, the inhibition of the experimental activation of lysozyme and phagocytosis, and of wound healing in the polychaete Eurythoe complanata exposed to acute copper exposure, suggests the metal´  s potential toxicity on the physiological mechanisms that modulate the innate immunity in annelids, whose alteration could affect their tolerance to microbial infection.
format	info:eu-repo/semantics/article
author	Zapata-Vívenes, E Nusetti, OA Marcano, L Esclapés, MM Arredondo, L
author_facet	Zapata-Vívenes, E Nusetti, OA Marcano, L Esclapés, MM Arredondo, L
author_sort	Zapata-Vívenes, E
title	Immunological responses and wound healing in the polychaete Eurythoe complanata (Annelida: Amphinomidae) exposed to copper
title_short	Immunological responses and wound healing in the polychaete Eurythoe complanata (Annelida: Amphinomidae) exposed to copper
title_full	Immunological responses and wound healing in the polychaete Eurythoe complanata (Annelida: Amphinomidae) exposed to copper
title_fullStr	Immunological responses and wound healing in the polychaete Eurythoe complanata (Annelida: Amphinomidae) exposed to copper
title_full_unstemmed	Immunological responses and wound healing in the polychaete Eurythoe complanata (Annelida: Amphinomidae) exposed to copper
title_sort	immunological responses and wound healing in the polychaete eurythoe complanata (annelida: amphinomidae) exposed to copper
publisher	Iniversidad Autónoma de Baja California
publishDate	2005
url	https://www.cienciasmarinas.com.mx/index.php/cmarinas/article/view/83
_version_	1792608895375507456

Immunological responses and wound healing in the polychaete Eurythoe complanata (Annelida: Amphinomidae) exposed to copper

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