Interlaboratory assessment of marine bioassays to evaluate the environmental quality of coastal sediments in Spain. II. Bioluminescence inhibition test for rapid sediment toxicity assessment

Interlaboratory assessment of marine bioassays to evaluate the environmental quality of coastal sediments in Spain. II. Bioluminescence inhibition test for rapid sediment toxicity assessment

The Microtox® bioassay was tested in an interlaboratory study to evaluate the variability when using solid-phase samples. The exercise consisted of two consecutive phases each one carried out with six sediment samples from Spanish ports. Phase I included six laboratories that reported results for th...

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Główni autorzy: Casado-Martínez, Mc, Campisi, T, Díaz, A, Lo-Re, R, Obispo, R, Postma, JF, Riba, I, Sneekes, AC, Buceta, JL, del-Valls, TA
Format: info:eu-repo/semantics/article
Język:eng
Wydane: Iniversidad Autónoma de Baja California 2006
Hasła przedmiotowe:
Microtox®
round robin test,
dredged material
Vibrio fischeri
material de dragado
variabilidad interlaboratorio
Dostęp online:https://www.cienciasmarinas.com.mx/index.php/cmarinas/article/view/1031
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spelling repositorioinstitucional-20.500.12930-73892023-05-09T14:30:58Z Interlaboratory assessment of marine bioassays to evaluate the environmental quality of coastal sediments in Spain. II. Bioluminescence inhibition test for rapid sediment toxicity assessment Ejercicio interlaboratorio de bioensayos marinos para la evaluación de la calidad ambiental de sedimentos costeros en España. II. Ensayo de inhibición de la bioluminiscencia para la evaluación rápida de la toxicidad de sedimentos Casado-Martínez, Mc Campisi, T Díaz, A Lo-Re, R Obispo, R Postma, JF Riba, I Sneekes, AC Buceta, JL del-Valls, TA Microtox® round robin test, dredged material Vibrio fischeri Microtox® material de dragado Vibrio fischeri variabilidad interlaboratorio The Microtox® bioassay was tested in an interlaboratory study to evaluate the variability when using solid-phase samples. The exercise consisted of two consecutive phases each one carried out with six sediment samples from Spanish ports. Phase I included six laboratories that reported results for the solid-phase test (SPT) protocol, while Phase II included seven laboratories for the SPT protocol and three laboratories for the basic solid-phase test (BSPT) protocol. Even though some interfering factors were identified that could affect the results and sample classification, the coefficients of variation (CV) can be considered in the range for biological tests. When these factors are considered for further assessments, interlaboratory variability is significantly reduced. The BSPT showed lower CV values than the SPT despite the small number of participating laboratories. This better performance may be explained either by the simplicity of the methodology. El presente trabajo resume el ejercicio interlaboratorio realizado para evaluar la variabilidad del ensayo Microtox® sobre fase sólida. El ejercicio se dividió en dos fases consecutivas, cada una de ellas desarrollada sobre seis muestras de sedimento de puertos españoles. La Fase I incluyó seis laboratorios y se obtuvieron resultados para el protocolo SPT (Solid Phase Test). La Fase II incluyó siete laboratorios y, además de obtenerse resultados del protocolo SPT, tres de ellos utilizaron también el protocolo para la fase sólida BSPT (Basic Solid Phase Test). Si bien se han identificado factores que pueden tener un cierto efecto sobre los resultados, y por lo tanto en la clasificación de las muestras, los coeficientes de variación obtenidos pueden considerarse entre los valores normales para ensayos biológicos. Si esto se tiene en cuenta para el análisis de los resultados, especialmente para los de la Fase I, la variabilidad baja considerablemente. El protocolo BSPT obtuvo resultados más consistentes que el protocolo SPT aun contando sólo con los resultados de un número reducido de laboratorios. La mayor simplicidad del ensayo pudo ser la causa de esta menor variabilidad.  2006-05-24 2021-06-03T03:55:08Z 2021-06-03T03:55:08Z info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed research note Nota de investigación arbitrada https://www.cienciasmarinas.com.mx/index.php/cmarinas/article/view/1031 10.7773/cm.v32i12.1031 https://hdl.handle.net/20.500.12930/7389 eng https://www.cienciasmarinas.com.mx/index.php/cmarinas/article/view/1031/934 application/pdf Iniversidad Autónoma de Baja California Ciencias Marinas; Vol. 32 No. 1B (2006); 129-138 Ciencias Marinas; Vol. 32 Núm. 1B (2006); 129-138 2395-9053 0185-3880
institution Repositorio Institucional
collection DSpace
language eng
topic Microtox®
round robin test,
dredged material
Vibrio fischeri
Microtox®
material de dragado
Vibrio fischeri
variabilidad interlaboratorio
spellingShingle Microtox®
round robin test,
dredged material
Vibrio fischeri
Microtox®
material de dragado
Vibrio fischeri
variabilidad interlaboratorio
Casado-Martínez, Mc
Campisi, T
Díaz, A
Lo-Re, R
Obispo, R
Postma, JF
Riba, I
Sneekes, AC
Buceta, JL
del-Valls, TA
Interlaboratory assessment of marine bioassays to evaluate the environmental quality of coastal sediments in Spain. II. Bioluminescence inhibition test for rapid sediment toxicity assessment
description The Microtox® bioassay was tested in an interlaboratory study to evaluate the variability when using solid-phase samples. The exercise consisted of two consecutive phases each one carried out with six sediment samples from Spanish ports. Phase I included six laboratories that reported results for the solid-phase test (SPT) protocol, while Phase II included seven laboratories for the SPT protocol and three laboratories for the basic solid-phase test (BSPT) protocol. Even though some interfering factors were identified that could affect the results and sample classification, the coefficients of variation (CV) can be considered in the range for biological tests. When these factors are considered for further assessments, interlaboratory variability is significantly reduced. The BSPT showed lower CV values than the SPT despite the small number of participating laboratories. This better performance may be explained either by the simplicity of the methodology.
format info:eu-repo/semantics/article
author Casado-Martínez, Mc
Campisi, T
Díaz, A
Lo-Re, R
Obispo, R
Postma, JF
Riba, I
Sneekes, AC
Buceta, JL
del-Valls, TA
author_facet Casado-Martínez, Mc
Campisi, T
Díaz, A
Lo-Re, R
Obispo, R
Postma, JF
Riba, I
Sneekes, AC
Buceta, JL
del-Valls, TA
author_sort Casado-Martínez, Mc
title Interlaboratory assessment of marine bioassays to evaluate the environmental quality of coastal sediments in Spain. II. Bioluminescence inhibition test for rapid sediment toxicity assessment
title_short Interlaboratory assessment of marine bioassays to evaluate the environmental quality of coastal sediments in Spain. II. Bioluminescence inhibition test for rapid sediment toxicity assessment
title_full Interlaboratory assessment of marine bioassays to evaluate the environmental quality of coastal sediments in Spain. II. Bioluminescence inhibition test for rapid sediment toxicity assessment
title_fullStr Interlaboratory assessment of marine bioassays to evaluate the environmental quality of coastal sediments in Spain. II. Bioluminescence inhibition test for rapid sediment toxicity assessment
title_full_unstemmed Interlaboratory assessment of marine bioassays to evaluate the environmental quality of coastal sediments in Spain. II. Bioluminescence inhibition test for rapid sediment toxicity assessment
title_sort interlaboratory assessment of marine bioassays to evaluate the environmental quality of coastal sediments in spain. ii. bioluminescence inhibition test for rapid sediment toxicity assessment
publisher Iniversidad Autónoma de Baja California
publishDate 2006
url https://www.cienciasmarinas.com.mx/index.php/cmarinas/article/view/1031
_version_ 1792610585359155200

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