Enzymatic activity of acid and alkaline phosphatase and catalase in Ruditapes philippinarum as biomarkers of stress caused by anionic (C11-LAS) and non-ionic (NPEO2.8) surfactants

Enzymatic activity of acid and alkaline phosphatase and catalase in Ruditapes philippinarum as biomarkers of stress caused by anionic (C11-LAS) and non-ionic (NPEO2.8) surfactants

 A subchronic toxicity study was performed by exposing organisms to two surfactants in a continuous flow-through system for 120 h, followed by a depuration phase of 48 h. The bivalve mollusc Ruditapes philippinarum, widely distributed in intertidal zones and of high commercial value, was us...

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Główni autorzy: Álvarez-Muñoz, D, Sáez, M, Blasco, J, Gómez-Parra, A, González-Mazo, E
Format: info:eu-repo/semantics/article
Język:eng
Wydane: Iniversidad Autónoma de Baja California 2006
Hasła przedmiotowe:
surfactant
acid phosphatase
alkaline phosphatase
catalase
Ruditapes philippinarum
tensioactivo
fosfatasa ácida
fosfatasa alcalina
catalasa
Dostęp online:https://www.cienciasmarinas.com.mx/index.php/cmarinas/article/view/1082
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id repositorioinstitucional-20.500.12930-7409
record_format dspace
institution Repositorio Institucional
collection DSpace
language eng
topic surfactant
acid phosphatase
alkaline phosphatase
catalase
Ruditapes philippinarum
tensioactivo
fosfatasa ácida
fosfatasa alcalina
catalasa
Ruditapes philippinarum
spellingShingle surfactant
acid phosphatase
alkaline phosphatase
catalase
Ruditapes philippinarum
tensioactivo
fosfatasa ácida
fosfatasa alcalina
catalasa
Ruditapes philippinarum
Álvarez-Muñoz, D
Sáez, M
Blasco, J
Gómez-Parra, A
González-Mazo, E
Enzymatic activity of acid and alkaline phosphatase and catalase in Ruditapes philippinarum as biomarkers of stress caused by anionic (C11-LAS) and non-ionic (NPEO2.8) surfactants
description  A subchronic toxicity study was performed by exposing organisms to two surfactants in a continuous flow-through system for 120 h, followed by a depuration phase of 48 h. The bivalve mollusc Ruditapes philippinarum, widely distributed in intertidal zones and of high commercial value, was used. The main anionic and non-ionic surfactants employed were linear alkylbenzene sulphonate (LAS) and nonylphenol ethoxylate (NPEO2.8), respectively. The organisms were exposed to three environmental levels (µg L–1), and the concentrations of C11-LAS measured in the tissues were 2.4, 6.7 and 12.8 µg g–1 for the low, intermediate and high exposure levels, while those of NPEO2.8 were 13.4, 14.8 and 31.7 µg g–1, respectively. The responses used as "end point" of subcellular toxicity were the enzymatic activity of catalase (CAT), acid phosphatase (AcP) and alkaline phosphatase (ALP). No significant differences were observed in CAT activity at the end of the exposure phase; however, at the end of the depuration phase there was an increase in CAT activity in the organisms exposed to the low concentration of C11-LAS (P < 0.05) and a significant decrease in the individuals exposed to the high concentration of NPEO2.8, which is related to the largest accumulation of the compound inside the organism. The organisms exposed to the intermediate and high concentrations (P < 0.05) of C11-LAS showed inhibition for AcP and activation for ALP at the end of the experiment (day 7). In the organisms exposed to the high concentration of NPEO2.8 there was an increase in the specific activity at the end of the depuration stage.
format info:eu-repo/semantics/article
author Álvarez-Muñoz, D
Sáez, M
Blasco, J
Gómez-Parra, A
González-Mazo, E
author_facet Álvarez-Muñoz, D
Sáez, M
Blasco, J
Gómez-Parra, A
González-Mazo, E
author_sort Álvarez-Muñoz, D
title Enzymatic activity of acid and alkaline phosphatase and catalase in Ruditapes philippinarum as biomarkers of stress caused by anionic (C11-LAS) and non-ionic (NPEO2.8) surfactants
title_short Enzymatic activity of acid and alkaline phosphatase and catalase in Ruditapes philippinarum as biomarkers of stress caused by anionic (C11-LAS) and non-ionic (NPEO2.8) surfactants
title_full Enzymatic activity of acid and alkaline phosphatase and catalase in Ruditapes philippinarum as biomarkers of stress caused by anionic (C11-LAS) and non-ionic (NPEO2.8) surfactants
title_fullStr Enzymatic activity of acid and alkaline phosphatase and catalase in Ruditapes philippinarum as biomarkers of stress caused by anionic (C11-LAS) and non-ionic (NPEO2.8) surfactants
title_full_unstemmed Enzymatic activity of acid and alkaline phosphatase and catalase in Ruditapes philippinarum as biomarkers of stress caused by anionic (C11-LAS) and non-ionic (NPEO2.8) surfactants
title_sort enzymatic activity of acid and alkaline phosphatase and catalase in ruditapes philippinarum as biomarkers of stress caused by anionic (c11-las) and non-ionic (npeo2.8) surfactants
publisher Iniversidad Autónoma de Baja California
publishDate 2006
url https://www.cienciasmarinas.com.mx/index.php/cmarinas/article/view/1082
_version_ 1792609854289870848
spelling repositorioinstitucional-20.500.12930-74092023-05-09T14:30:50Z Enzymatic activity of acid and alkaline phosphatase and catalase in Ruditapes philippinarum as biomarkers of stress caused by anionic (C11-LAS) and non-ionic (NPEO2.8) surfactants Actividades enzimáticas de las fosfatasas ácida y alcalina y la catalasa en Ruditapes philippinarum como biomarcadores del estrés generado por tensioactivos aniónicos (C11-LAS) y no iónicos (NPEO2.8) Álvarez-Muñoz, D Sáez, M Blasco, J Gómez-Parra, A González-Mazo, E surfactant acid phosphatase alkaline phosphatase catalase Ruditapes philippinarum tensioactivo fosfatasa ácida fosfatasa alcalina catalasa Ruditapes philippinarum  A subchronic toxicity study was performed by exposing organisms to two surfactants in a continuous flow-through system for 120 h, followed by a depuration phase of 48 h. The bivalve mollusc Ruditapes philippinarum, widely distributed in intertidal zones and of high commercial value, was used. The main anionic and non-ionic surfactants employed were linear alkylbenzene sulphonate (LAS) and nonylphenol ethoxylate (NPEO2.8), respectively. The organisms were exposed to three environmental levels (µg L–1), and the concentrations of C11-LAS measured in the tissues were 2.4, 6.7 and 12.8 µg g–1 for the low, intermediate and high exposure levels, while those of NPEO2.8 were 13.4, 14.8 and 31.7 µg g–1, respectively. The responses used as "end point" of subcellular toxicity were the enzymatic activity of catalase (CAT), acid phosphatase (AcP) and alkaline phosphatase (ALP). No significant differences were observed in CAT activity at the end of the exposure phase; however, at the end of the depuration phase there was an increase in CAT activity in the organisms exposed to the low concentration of C11-LAS (P < 0.05) and a significant decrease in the individuals exposed to the high concentration of NPEO2.8, which is related to the largest accumulation of the compound inside the organism. The organisms exposed to the intermediate and high concentrations (P < 0.05) of C11-LAS showed inhibition for AcP and activation for ALP at the end of the experiment (day 7). In the organisms exposed to the high concentration of NPEO2.8 there was an increase in the specific activity at the end of the depuration stage. Se realizó un estudio de toxicidad subcrónica mediante la exposición de organismos a dos tensioactivos en un sistema en flujo continuo, con un periodo de exposición al tóxico de 120 h, y otro de depuración en ausencia del mismo de 48 h. Los experimentos se realizaron con Ruditapes philippinarum, molusco con amplia distribución en zonas intermareales y de gran importancia económica, utilizando sulfonato de alquilbenceno lineal (LAS) como principal tensioactivo aniónico y el nonilfenol etoxilado (NPEO2.8) como no iónico. Los organismos fueron expuestos a tres concentraciones ambientales (µg L–1) de LAS y de NPEO2.8, siendo 2.4, 6.7 y 12.8 µg g–1 de LAS las concentraciones medidas en el interior de organismos expuestos a la concentración menor, intermedia y mayor, mientras que las concentraciones de NPEO2.8 fueron de 13.4 y 14.8 y 31.7 µg g–1, respectivamente. Las respuestas empleadas como "criterio de efecto" de toxicidad subcelular fueron: las medidas de las actividades enzimáticas de la catalasa (CAT), y las fosfatasas ácida (AcP) y alcalina (ALP). En el caso de CAT, no se han observado diferencias significativas al final de la fase de exposición con ninguno de ambos tensioactivos. Sin embargo, al final de la fase de depuración existió un aumento de la actividad CAT con el C11LAS en los organismos expuestos a la menor concentración (P < 0.05) y una disminución significativa con el NPEO2.8 en los organismos expuestos a la mayor concentración, la misma que corresponde con la mayor acumulación del compuesto en el interior del organismo. En los organismos expuestos a las concentraciones media y mayor de C11-LAS se observó inhibición de la actividad AcP y activación de la ALP al final del ensayo (día 7) (P < 0.05). En los organismos expuestos a la mayor concentración de NPEO2.8 existe un aumento de la actividad específica al final de la fase de depuración. 2006-08-21 2021-06-03T03:55:13Z 2021-06-03T03:55:13Z info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed research note Nota de investigación arbitrada https://www.cienciasmarinas.com.mx/index.php/cmarinas/article/view/1082 10.7773/cm.v32i22.1082 https://hdl.handle.net/20.500.12930/7409 eng https://www.cienciasmarinas.com.mx/index.php/cmarinas/article/view/1082/1925 application/pdf Iniversidad Autónoma de Baja California Ciencias Marinas; Vol. 32 No. 2B (2006); 447-455 Ciencias Marinas; Vol. 32 Núm. 2B (2006); 447-455 2395-9053 0185-3880

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