Extraction and purification of B-phycoerythrin from the red microalga Rhodosorus marinus

A description is given of the purification of B-phycoerythrin (B-PE) from the red microalga Rhodosorus marinus. Initially, phycobiliproteins were released from the microalgal cells by manual cellular fragmentation and sonication. B-PE was extracted with ammonium sulfate precipitation, and purified b...

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Hauptverfasser:	Básaca-Loya, GA, Valdez, MA, Enríquez-Guevara, EA, Gutierrez-Millán, LE, Burboa, MG
Format:	info:eu-repo/semantics/article
Sprache:	eng
Veröffentlicht:	Iniversidad Autónoma de Baja California 2009
Schlagworte:	microalgae phycobiliproteins phycoerythrin pigments Rhodosorus marinus microalgas ficobiliproteínas ficoeritrina pigmentos
Online Zugang:	https://www.cienciasmarinas.com.mx/index.php/cmarinas/article/view/1614
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id	repositorioinstitucional-20.500.12930-7425
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spelling	repositorioinstitucional-20.500.12930-74252023-05-09T14:30:49Z Extraction and purification of B-phycoerythrin from the red microalga Rhodosorus marinus Extracción y purificación de B-ficoeritrina de la microalga roja Rhodosorus marinus Básaca-Loya, GA Valdez, MA Enríquez-Guevara, EA Gutierrez-Millán, LE Burboa, MG microalgae phycobiliproteins phycoerythrin pigments Rhodosorus marinus microalgas ficobiliproteínas ficoeritrina pigmentos Rhodosorus marinus A description is given of the purification of B-phycoerythrin (B-PE) from the red microalga Rhodosorus marinus. Initially, phycobiliproteins were released from the microalgal cells by manual cellular fragmentation and sonication. B-PE was extracted with ammonium sulfate precipitation, and purified by anionic and size exclusion chromatography. Its purity was tested using indexes and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Spectroscopic characterization of B-PE was performed by UV-visible spectroscopy, fluorescence spectroscopy, and circular dichroism. Rhodosorus marinus showed three types of phycobiliproteins: phycoerythrin, phycocyanin, and allophycocyanin. The purified B-PE showed a purity ratio(A545/A280) of 4.8, characteristic peaks at 540 and 562 nm with a shoulder at 498 nm, fluorescence emission maximum at 578 nm, and a secondary structure almost stable with pH changes. B-PE was found to be the predominant pigment in R. marinus and this red microalga could be a viable option for the recovery of this chromoprotein. En este trabajo se describe la purificación de la proteína B-ficoeritrina (B-PE) de la microalga roja Rhodosorus marinus. Inicialmente se extrajeron las ficobiliproteínas de las células microalgales mediante fragmentación manual y sonicación. La BPE fue purificada mediante precipitación con sulfato de amonio y cromatografía de exclusión por tamaño e intercambio aniónico. Se determinó la pureza de B-PE por medio de índices y electroforesis en gel de poliacrilamida con dodecilsulfato de sodio. La caracterización espectroscópica de B-PE se realizó mediante espectroscopía UV-visible, espectroscopía de fluorescencia y dicroísmo circular. Rhodosorus marinus presentó tres tipos de ficobiliproteínas: ficoeritrina, ficocianina y aloficocianina. La B-PE purificada mostró un índice de pureza (A545/A280) de 4.8, picos característicos a 540 y 562 nm con un hombro a 498 nm, un máximo de emisión de fluorescencia de 578 nm, y una estructura secundaria casi estable ante cambios de pH. La B-PE mostró ser el pigmento predominante en R. marinus. Esta microalga roja podría ser una opción viable para la recuperación de esta cromoproteína. 2009-12-08 2021-06-03T03:55:16Z 2021-06-03T03:55:16Z info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article Artículo Arbitrado https://www.cienciasmarinas.com.mx/index.php/cmarinas/article/view/1614 10.7773/cm.v35i4.1614 https://hdl.handle.net/20.500.12930/7425 eng https://www.cienciasmarinas.com.mx/index.php/cmarinas/article/view/1614/1783 application/pdf Iniversidad Autónoma de Baja California Ciencias Marinas; Vol. 35 No. 4 (2009); 359-368 Ciencias Marinas; Vol. 35 Núm. 4 (2009); 359-368 2395-9053 0185-3880
institution	Repositorio Institucional
collection	DSpace
language	eng
topic	microalgae phycobiliproteins phycoerythrin pigments Rhodosorus marinus microalgas ficobiliproteínas ficoeritrina pigmentos Rhodosorus marinus
spellingShingle	microalgae phycobiliproteins phycoerythrin pigments Rhodosorus marinus microalgas ficobiliproteínas ficoeritrina pigmentos Rhodosorus marinus Básaca-Loya, GA Valdez, MA Enríquez-Guevara, EA Gutierrez-Millán, LE Burboa, MG Extraction and purification of B-phycoerythrin from the red microalga Rhodosorus marinus
description	A description is given of the purification of B-phycoerythrin (B-PE) from the red microalga Rhodosorus marinus. Initially, phycobiliproteins were released from the microalgal cells by manual cellular fragmentation and sonication. B-PE was extracted with ammonium sulfate precipitation, and purified by anionic and size exclusion chromatography. Its purity was tested using indexes and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Spectroscopic characterization of B-PE was performed by UV-visible spectroscopy, fluorescence spectroscopy, and circular dichroism. Rhodosorus marinus showed three types of phycobiliproteins: phycoerythrin, phycocyanin, and allophycocyanin. The purified B-PE showed a purity ratio(A545/A280) of 4.8, characteristic peaks at 540 and 562 nm with a shoulder at 498 nm, fluorescence emission maximum at 578 nm, and a secondary structure almost stable with pH changes. B-PE was found to be the predominant pigment in R. marinus and this red microalga could be a viable option for the recovery of this chromoprotein.
format	info:eu-repo/semantics/article
author	Básaca-Loya, GA Valdez, MA Enríquez-Guevara, EA Gutierrez-Millán, LE Burboa, MG
author_facet	Básaca-Loya, GA Valdez, MA Enríquez-Guevara, EA Gutierrez-Millán, LE Burboa, MG
author_sort	Básaca-Loya, GA
title	Extraction and purification of B-phycoerythrin from the red microalga Rhodosorus marinus
title_short	Extraction and purification of B-phycoerythrin from the red microalga Rhodosorus marinus
title_full	Extraction and purification of B-phycoerythrin from the red microalga Rhodosorus marinus
title_fullStr	Extraction and purification of B-phycoerythrin from the red microalga Rhodosorus marinus
title_full_unstemmed	Extraction and purification of B-phycoerythrin from the red microalga Rhodosorus marinus
title_sort	extraction and purification of b-phycoerythrin from the red microalga rhodosorus marinus
publisher	Iniversidad Autónoma de Baja California
publishDate	2009
url	https://www.cienciasmarinas.com.mx/index.php/cmarinas/article/view/1614
_version_	1792609854413602816

Extraction and purification of B-phycoerythrin from the red microalga Rhodosorus marinus

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