Pedigree traceability in whiteleg shrimp (Litopenaeus vannamei) using genetic markers: A comparison between microsatellites and SNPs

To increase productivity, genetic improvement in cultivated shrimp is of much interest. Evaluation of genetic parameters (e.g., heritability, genotype–environment interaction, inbreeding) and the design of appropriate breeding plans are necessary steps towards genetic improvement. Pedigree traceabil...

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Main Authors: Perez-Enriquez, Ricardo, Max-Aguilar, Adriana
Format: info:eu-repo/semantics/article
Language:eng
Published: Iniversidad Autónoma de Baja California 2016
Online Access:https://www.cienciasmarinas.com.mx/index.php/cmarinas/article/view/2662
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spelling repositorioinstitucional-20.500.12930-75092023-05-09T14:30:29Z Pedigree traceability in whiteleg shrimp (Litopenaeus vannamei) using genetic markers: A comparison between microsatellites and SNPs Rastreabilidad del pedigrí en camarón blanco (Litopenaeus vannamei) mediante marcadores genéticos: Una comparación entre microsatélites y SNP Perez-Enriquez, Ricardo Max-Aguilar, Adriana To increase productivity, genetic improvement in cultivated shrimp is of much interest. Evaluation of genetic parameters (e.g., heritability, genotype–environment interaction, inbreeding) and the design of appropriate breeding plans are necessary steps towards genetic improvement. Pedigree traceability by genetic markers is relevant to these objectives. The aim of this study was to compare the performance of 2 genetic marker panels (microsatellites and single nucleotide polymorphisms [SNPs]) for pedigree traceability of a cultivated stock of whiteleg shrimp (Litopenaeus vannamei). Pedigree of a stock from 81 full-sib families reared in a common environment was assessed with microsatellites and SNPs as genetic markers. The panel of 76 SNPs performed better than microsatellites, allowing 94–96% of parentage assignment of the tested progeny (n = 192). A minimum number of 50 SNPs with a proportion of 60% loci with a minimum allele frequency of 0.3 is suitable for successful pedigree assignment. SNP markers are suggested for confidently testing the pedigree of shrimp from known parental broodstock.  El mejoramiento genético en el camarón cultivado es de amplio interés para incrementar la productividad. La evaluación de parámetros genéticos (e.g., heredabilidad, interacción entre genotipo y ambiente, endogamia) y el diseño de planes de apareamiento son elementos necesarios para el mejoramiento genético. La rastreabilidad del pedigrí con marcadores genéticos es importante para estos objetivos. El objetivo de este trabajo fue comparar el desempeño de 2 paneles de marcadores genéticos (microsatélites y polimorfismos de nucleótido sencillo [SNP]) para rastrear el pedigrí en una población de camarón blanco (Litopenaeus vannamei) de cultivo. Se determinó el pedigrí de la progenie de 81 familias de hermanos completos mediante el uso de microsatélites y SNP como marcadores genéticos. Un panel de 76 SNP probó tener un mejor desempeño que los microsatélites para la asignación de parentesco, obteniéndose un 94–96% de asignación en una muestra de la progenie (n = 192). Un número mínimo de 50 SNP con una proporción de 60% de loci con una frecuencia alélica mínima de 0.3 es adecuado para una asignación exitosa de pedigrí. Se sugiere utilizar los marcadores SNP para determinar el parentesco de la progenie de camarón proveniente de padres conocidos. 2016-12-20 2021-06-03T03:55:32Z 2021-06-03T03:55:32Z info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article Artículo Arbitrado https://www.cienciasmarinas.com.mx/index.php/cmarinas/article/view/2662 10.7773/cm.v42i4.2662 https://hdl.handle.net/20.500.12930/7509 eng https://www.cienciasmarinas.com.mx/index.php/cmarinas/article/view/2662/1607 Copyright (c) 2016 Ciencias Marinas application/pdf Iniversidad Autónoma de Baja California Ciencias Marinas; Vol. 42 No. 4 (2016); 227–235 Ciencias Marinas; Vol. 42 Núm. 4 (2016); 227–235 2395-9053 0185-3880
institution Repositorio Institucional
collection DSpace
language eng
description To increase productivity, genetic improvement in cultivated shrimp is of much interest. Evaluation of genetic parameters (e.g., heritability, genotype–environment interaction, inbreeding) and the design of appropriate breeding plans are necessary steps towards genetic improvement. Pedigree traceability by genetic markers is relevant to these objectives. The aim of this study was to compare the performance of 2 genetic marker panels (microsatellites and single nucleotide polymorphisms [SNPs]) for pedigree traceability of a cultivated stock of whiteleg shrimp (Litopenaeus vannamei). Pedigree of a stock from 81 full-sib families reared in a common environment was assessed with microsatellites and SNPs as genetic markers. The panel of 76 SNPs performed better than microsatellites, allowing 94–96% of parentage assignment of the tested progeny (n = 192). A minimum number of 50 SNPs with a proportion of 60% loci with a minimum allele frequency of 0.3 is suitable for successful pedigree assignment. SNP markers are suggested for confidently testing the pedigree of shrimp from known parental broodstock. 
format info:eu-repo/semantics/article
author Perez-Enriquez, Ricardo
Max-Aguilar, Adriana
spellingShingle Perez-Enriquez, Ricardo
Max-Aguilar, Adriana
Pedigree traceability in whiteleg shrimp (Litopenaeus vannamei) using genetic markers: A comparison between microsatellites and SNPs
author_facet Perez-Enriquez, Ricardo
Max-Aguilar, Adriana
author_sort Perez-Enriquez, Ricardo
title Pedigree traceability in whiteleg shrimp (Litopenaeus vannamei) using genetic markers: A comparison between microsatellites and SNPs
title_short Pedigree traceability in whiteleg shrimp (Litopenaeus vannamei) using genetic markers: A comparison between microsatellites and SNPs
title_full Pedigree traceability in whiteleg shrimp (Litopenaeus vannamei) using genetic markers: A comparison between microsatellites and SNPs
title_fullStr Pedigree traceability in whiteleg shrimp (Litopenaeus vannamei) using genetic markers: A comparison between microsatellites and SNPs
title_full_unstemmed Pedigree traceability in whiteleg shrimp (Litopenaeus vannamei) using genetic markers: A comparison between microsatellites and SNPs
title_sort pedigree traceability in whiteleg shrimp (litopenaeus vannamei) using genetic markers: a comparison between microsatellites and snps
publisher Iniversidad Autónoma de Baja California
publishDate 2016
url https://www.cienciasmarinas.com.mx/index.php/cmarinas/article/view/2662
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