Analysis of antioxidant capacity and bioactive compounds in marine macroalgal and lichenic extracts using different solvents and evaluation methods

Analysis of antioxidant capacity and bioactive compounds in marine macroalgal and lichenic extracts using different solvents and evaluation methods

Natural extracts of macroalgae are widely recognized for their antioxidant properties. In this work, the antioxidant capacity of various aqueous and hydroalcoholic extracts obtained from red and green marine algae and from one marine lichen collected from different sites in southern Spain (intertida...

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Autores principales: Álvarez-Gomez, Felix, Korbee, Nathalie, Figueroa, Felix L
Formato: info:eu-repo/semantics/article
Lenguaje:eng
Publicado: Iniversidad Autónoma de Baja California 2016
Acceso en línea:https://www.cienciasmarinas.com.mx/index.php/cmarinas/article/view/2677
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language eng
description Natural extracts of macroalgae are widely recognized for their antioxidant properties. In this work, the antioxidant capacity of various aqueous and hydroalcoholic extracts obtained from red and green marine algae and from one marine lichen collected from different sites in southern Spain (intertidal and estuarine waters) was evaluated by different methods: ABTS free radical scavenging assay, DPPH assay, and beta-carotene bleaching method (BBM). Contents of total lipids, total carbohydrates, and UV photoprotectors with antioxidant capacity, such as mycosporine-like amino acids and phenolic compounds, were determined. Among the extraction solvents, the highest extraction yield was observed in H2O and 20% MeOH (v/v). The highest antioxidant activity was found in the extracts of the red macroalgae Hydropuntia cornea, Gracilariopsis longissima, Halopithys incurva, and Porphyra umbilicalis, whereas the lowest activity was detected in the green macroalga Ulva rotundata. In general, the antioxidant activity was higher using DPPH than BBM and ABTS. Even so, the ABTS assay is an easy and quick test that provides a comprehensive view of the entire extract in both the lipophilic medium and hydrophilic medium. The antioxidant activity was related to the composition of bioactive compounds and synergistic action is not discarded. The biotechnological use of macroalgal extracts with high antioxidant capacity is discussed. 
format info:eu-repo/semantics/article
author Álvarez-Gomez, Felix
Korbee, Nathalie
Figueroa, Felix L
spellingShingle Álvarez-Gomez, Felix
Korbee, Nathalie
Figueroa, Felix L
Analysis of antioxidant capacity and bioactive compounds in marine macroalgal and lichenic extracts using different solvents and evaluation methods
author_facet Álvarez-Gomez, Felix
Korbee, Nathalie
Figueroa, Felix L
author_sort Álvarez-Gomez, Felix
title Analysis of antioxidant capacity and bioactive compounds in marine macroalgal and lichenic extracts using different solvents and evaluation methods
title_short Analysis of antioxidant capacity and bioactive compounds in marine macroalgal and lichenic extracts using different solvents and evaluation methods
title_full Analysis of antioxidant capacity and bioactive compounds in marine macroalgal and lichenic extracts using different solvents and evaluation methods
title_fullStr Analysis of antioxidant capacity and bioactive compounds in marine macroalgal and lichenic extracts using different solvents and evaluation methods
title_full_unstemmed Analysis of antioxidant capacity and bioactive compounds in marine macroalgal and lichenic extracts using different solvents and evaluation methods
title_sort analysis of antioxidant capacity and bioactive compounds in marine macroalgal and lichenic extracts using different solvents and evaluation methods
publisher Iniversidad Autónoma de Baja California
publishDate 2016
url https://www.cienciasmarinas.com.mx/index.php/cmarinas/article/view/2677
_version_ 1792608799514689536
spelling repositorioinstitucional-20.500.12930-75102023-05-09T14:30:24Z Analysis of antioxidant capacity and bioactive compounds in marine macroalgal and lichenic extracts using different solvents and evaluation methods Análisis de la capacidad antioxidante y compuestos bioactivos en extractos macroalgales y liquénicos mediante la aplicación de diferentes solventes y métodos de evaluación Álvarez-Gomez, Felix Korbee, Nathalie Figueroa, Felix L Natural extracts of macroalgae are widely recognized for their antioxidant properties. In this work, the antioxidant capacity of various aqueous and hydroalcoholic extracts obtained from red and green marine algae and from one marine lichen collected from different sites in southern Spain (intertidal and estuarine waters) was evaluated by different methods: ABTS free radical scavenging assay, DPPH assay, and beta-carotene bleaching method (BBM). Contents of total lipids, total carbohydrates, and UV photoprotectors with antioxidant capacity, such as mycosporine-like amino acids and phenolic compounds, were determined. Among the extraction solvents, the highest extraction yield was observed in H2O and 20% MeOH (v/v). The highest antioxidant activity was found in the extracts of the red macroalgae Hydropuntia cornea, Gracilariopsis longissima, Halopithys incurva, and Porphyra umbilicalis, whereas the lowest activity was detected in the green macroalga Ulva rotundata. In general, the antioxidant activity was higher using DPPH than BBM and ABTS. Even so, the ABTS assay is an easy and quick test that provides a comprehensive view of the entire extract in both the lipophilic medium and hydrophilic medium. The antioxidant activity was related to the composition of bioactive compounds and synergistic action is not discarded. The biotechnological use of macroalgal extracts with high antioxidant capacity is discussed.  Los extractos naturales de las macroalgas son ampliamente reconocidos por sus propiedades antioxidantes. En este trabajo la capacidad antioxidante de diversos extractos acuosos e hidroalcohólicos obtenidos de algas marinas (rojas y verdes) y de un liquen marino, recolectados en diferentes puntos del sur de España (aguas intermareales y estuarinas), fue evaluada mediante diferentes métodos: método ABTS, ensayo DPPH y método de decoloración del beta-caroteno (BBM). Se determinó el contenido de lípidos totales, carbohidratos totales y fotoprotectores de radiación UV con capacidad antioxidante como los aminoácidos de tipo micosporina y compuestos fenólicos. Entre los solventes de extracción, el mayor rendimiento de extracción se observó en los solventes H2O y MeOH 20% (v/v). La mayor actividad antioxidante se encontró en los extractos de las macroalgas rojas Hydropuntia cornea, Gracilariopsis longissima, Halopithys incurva y Porphyra umbilicalis, mientras que la actividad más baja se detectó en los de la macroalga verde Ulva rotundata. En general, la actividad antioxidante fue mayor usando el ensayo DPPH que el método ABTS y el BBM. Aun así, se propone el método ABTS como una prueba sencilla y rápida que proporciona una visión completa del extracto en su conjunto, ya que mediante esta metodología se evalúan los componentes que se encuentran tanto en el medio lipofílico como el medio hidrofílico. La actividad antioxidante se relacionó con la composición de compuestos bioactivos sin descartar la acción sinérgica entre sus compuestos. Se discute el uso biotecnológico de los extractos de macroalgas con alta capacidad antioxidante. 2016-12-20 2021-06-03T03:55:32Z 2021-06-03T03:55:32Z info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article Artículo Arbitrado https://www.cienciasmarinas.com.mx/index.php/cmarinas/article/view/2677 10.7773/cm.v42i4.2677 https://hdl.handle.net/20.500.12930/7510 eng https://www.cienciasmarinas.com.mx/index.php/cmarinas/article/view/2677/1610 Copyright (c) 2016 Ciencias Marinas application/pdf Iniversidad Autónoma de Baja California Ciencias Marinas; Vol. 42 No. 4 (2016); 271–288 Ciencias Marinas; Vol. 42 Núm. 4 (2016); 271–288 2395-9053 0185-3880

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