Microaerophilic bacterial biomass measurements based on Adenosine Triphosphate and direct counting techniques in a semi-solid agar column

Microaerophilic bacterial biomass measurements based on Adenosine Triphosphate and direct counting techniques in a semi-solid agar column

Microaerophilic-diazotrophic bacterial biomass was measured by Adenosine Triphosphate (ATP) extraction and direct counting/biovolume techniques. Microaerophilic bacterial growth in a semi-solid agar column is visible in the form of sharp, well-defined growth rings at different depths within the colu...

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Autores principales: Ferrara-Guerrero, MJ, Bianchi, A
Formáid: Online
Teanga:eng
Foilsithe: Iniversidad Autónoma de Baja California 2000
Rochtain Ar Líne:https://www.cienciasmarinas.com.mx/index.php/cmarinas/article/view/575
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spelling oai:cienciasmarinas.com.mx:article-5752019-05-08T23:10:58Z Microaerophilic bacterial biomass measurements based on Adenosine Triphosphate and direct counting techniques in a semi-solid agar column Biomasa bacteriana microaerofílica calculada por las técnicas de Adenosina Trifosfato y de conteo directo en una columna de agar semisólido Ferrara-Guerrero, MJ Bianchi, A microaerophilic bacteria biomass ATP direct counting bacterias microaerofílicas biomasa ATP conteo directo Microaerophilic-diazotrophic bacterial biomass was measured by Adenosine Triphosphate (ATP) extraction and direct counting/biovolume techniques. Microaerophilic bacterial growth in a semi-solid agar column is visible in the form of sharp, well-defined growth rings at different depths within the column according to oxygen requirements. The microaerophilic zone is found between 3 and 15 mm below the agar surface, where the dissolved oxygen concentration varies from 4 to 172 µM. Biomass measurements calculated using a conventional conversion factor C/ATP of 250 were around ten times lower than those calculated according to the direct counting/biovolume technique. In the present study, we propose a conversion factor of 356 µg C/mL ATP that gives biomass measurements nearer to those obtained by direct counting. The highest bacterial metabolic activity was found between 5 and 10 mm depth (0.15 ± 0.029 µg ATP mL–1), coinciding with optimum oxygen concentration levels for microaerophilic growth (6.5 to 114 µM). La biomasa bacteriana microaerofílica-diazotrofa fue evaluada por las técnicas de extracción de Adenosina Trifosfato (ATP) y de conteo directo/biovolumen. El crecimiento bacteriano microaerofílico en columnas de agar semisólido se manifiesta en forma de anillos de crecimiento bien delimitados que se revelan a diferentes profundidades de la columna según sus necesidades de oxígeno. La zona microaerofílica se encuentra comprendida entre 3 y 15 mm por debajo de la superficie del agar, donde la concentración de oxígeno disuelto fluctúa entre 4 y 172 µM. Los valores de biomasa calculados utilizando un factor de conversión C/ATP convencional de 250 fueron aproximadamente diez veces menores que los calculados por el método de conteo directo/biovolumen. Durante esta investigación, se calculó un coeficiente de conversión de 356 µg C/mL ATP que dio valores de biomasa más cercanos a los obtenidos por conteo directo. La mayor actividad metabólica bacteriana se encontró entre 5 y 10 mm de profundidad (0.15 ± 0.029 µg ATP mL–1), coincidiendo con la zona con concentraciones óptimas de oxígeno para el crecimiento microaerofílico (6.5 a 14 µM). Iniversidad Autónoma de Baja California 2000-03-06 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article Artículo Arbitrado application/pdf https://www.cienciasmarinas.com.mx/index.php/cmarinas/article/view/575 10.7773/cm.v26i2.575 Ciencias Marinas; Vol. 26 No. 2 (2000); 355-367 Ciencias Marinas; Vol. 26 Núm. 2 (2000); 355-367 2395-9053 0185-3880 eng https://www.cienciasmarinas.com.mx/index.php/cmarinas/article/view/575/512
institution Ciencias Marinas
collection OJS
language eng
format Online
author Ferrara-Guerrero, MJ
Bianchi, A
spellingShingle Ferrara-Guerrero, MJ
Bianchi, A
Microaerophilic bacterial biomass measurements based on Adenosine Triphosphate and direct counting techniques in a semi-solid agar column
author_facet Ferrara-Guerrero, MJ
Bianchi, A
author_sort Ferrara-Guerrero, MJ
title Microaerophilic bacterial biomass measurements based on Adenosine Triphosphate and direct counting techniques in a semi-solid agar column
title_short Microaerophilic bacterial biomass measurements based on Adenosine Triphosphate and direct counting techniques in a semi-solid agar column
title_full Microaerophilic bacterial biomass measurements based on Adenosine Triphosphate and direct counting techniques in a semi-solid agar column
title_fullStr Microaerophilic bacterial biomass measurements based on Adenosine Triphosphate and direct counting techniques in a semi-solid agar column
title_full_unstemmed Microaerophilic bacterial biomass measurements based on Adenosine Triphosphate and direct counting techniques in a semi-solid agar column
title_sort microaerophilic bacterial biomass measurements based on adenosine triphosphate and direct counting techniques in a semi-solid agar column
description Microaerophilic-diazotrophic bacterial biomass was measured by Adenosine Triphosphate (ATP) extraction and direct counting/biovolume techniques. Microaerophilic bacterial growth in a semi-solid agar column is visible in the form of sharp, well-defined growth rings at different depths within the column according to oxygen requirements. The microaerophilic zone is found between 3 and 15 mm below the agar surface, where the dissolved oxygen concentration varies from 4 to 172 µM. Biomass measurements calculated using a conventional conversion factor C/ATP of 250 were around ten times lower than those calculated according to the direct counting/biovolume technique. In the present study, we propose a conversion factor of 356 µg C/mL ATP that gives biomass measurements nearer to those obtained by direct counting. The highest bacterial metabolic activity was found between 5 and 10 mm depth (0.15 ± 0.029 µg ATP mL–1), coinciding with optimum oxygen concentration levels for microaerophilic growth (6.5 to 114 µM).
publisher Iniversidad Autónoma de Baja California
publishDate 2000
url https://www.cienciasmarinas.com.mx/index.php/cmarinas/article/view/575
_version_ 1715723985200611328

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